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Sericin conductive hydrogel, preparation method thereof and scaffold prepared by sericin conductive hydrogel

A conductive hydrogel and sericin technology, which is used in pharmaceutical formulations, medical science, prostheses, etc. to achieve excellent electrical conductivity, reduce infection risk, and reduce trauma and pain.

Active Publication Date: 2016-12-07
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the existing hydrogel materials in this field has good electrical conductivity, memory deformation ability and biological activity at the same time.

Method used

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  • Sericin conductive hydrogel, preparation method thereof and scaffold prepared by sericin conductive hydrogel
  • Sericin conductive hydrogel, preparation method thereof and scaffold prepared by sericin conductive hydrogel
  • Sericin conductive hydrogel, preparation method thereof and scaffold prepared by sericin conductive hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0047] The preparation of embodiment 1-4 sericin conductive hydrogel

[0048] The preparation process of the sericin conductive hydrogel of the present embodiment is as follows: figure 1 shown.

[0049] 1. Preparation of sericin aqueous solution

[0050] The silk used in this example comes from the silkworm cocoon of the silkworm silk fibroin-deficient mutant (purchased from the Institute of Sericulture, Chinese Academy of Agricultural Sciences). An aqueous solution of sericin was prepared from the cocoons by the following method:

[0051] 1) Weigh 1 g of silkworm cocoon of mutant silkworm and cut it into 1 cm 2 The fragments were placed in a clean beaker, washed 3 times with ultrapure water, and centrifuged at 3500rpm for 5 minutes to remove water;

[0052] 2) Add 55 mL of LiBr aqueous solution with a concentration of 6 mol / L to the silkworm cocoon fragments obtained in step 1), put the beaker into a constant temperature water bath at 35° C. for 24 hours to dissolve sericin...

Embodiment 9-12

[0121] The preparation of embodiment 9-12 horseradish peroxidase (HRP) sustained-release agent

[0122] During the preparation of Example 1-4, the HRP solution was added so that the content of HRP in the final scaffold was 8 μg; the rest of the operations were the same as the preparation of the hydrogel in Example 1-4, thus obtaining four kinds of hydrogels containing HRP. These four sericin conductive hydrogels containing HRP were freeze-dried by the scaffold preparation method in Examples 5-8 to form the HRP slow-release agents of Examples 9-12.

[0123] Put the above slow-release agent in 1 mL of PBS with pH 7.4 at 37°C; , 264, 288, and 323 hours, take out the supernatant carefully, and add 1mL PBS (pH7.4) again; use enzyme-linked immunosorbent assay (ELISA) to measure the content of HRP in the supernatant, and calculate the accumulated HRP in the supernatant at each time point The ratio of the HRP content and the actual drug load is the cumulative release rate, the result...

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Abstract

The invention relates to sericin conductive hydrogel crosslinked by sericin protein. The sericin conductive hydrogel comprises 0.0125-0.0833g / mL sericin protein and 0.01-0.1mg / mL carbon nanotube. The invention further discloses a preparation method of the sericin conductive hydrogel, a scaffold prepared by the sericin conductive hydrogel and application of the scaffold. The sericin conductive hydrogel and the scaffold prepared by the sericin conductive hydrogel have excellent electrical conduction property, syringeability and memory deformation property and have nerve cell differentiation promoting ability, so that the sericin conductive hydrogel and the scaffold are especially applicable to the wound repairing and treatment of tissue, closely related to electrophysiological activities, of the nerves, the heart, the muscles and the like. The sericin conductive hydrogel and the scaffold and slow-release pharmaceutic preparation prepared by the sericin conductive hydrogel can be transplanted into a human body through injection or minimally invasive surgery, the wound and pain, caused by transplantation, of a patient is reduced to the maximum extent, infection risk caused by the transplantation is also lowered, and severe immunoreaction after the scaffold and the slow-release pharmaceutic preparation are transplanted into the human body is avoided.

Description

technical field [0001] The invention relates to the field of biomaterials, more particularly, to a sericin conductive hydrogel, a preparation method thereof and a scaffold prepared therefrom. Background technique [0002] Hydrogel (Hydrogel) is a gel with water as the dispersion medium. There are hydrophobic groups and hydrophilic groups in the water-soluble polymer with network crosslinked structure, and the hydrophilic groups combine with water molecules to bind water molecules It is a cross-linked polymer that is connected inside the network and the hydrophobic groups swell with water. [0003] Sericin (Silk Sericin) is a natural macromolecular viscous protein wrapped on the surface of silk fibers, accounting for about 20-30% of the cocoon content, composed of polypeptides with a molecular weight of 24-400kDa, and its molecules are composed of serine, asparagus It is composed of 18 kinds of amino acids such as amino acid and glycine. In recent years, sericin has become ...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08L89/00C08K7/24A61L27/54A61L27/50A61L27/22A61L27/08A61L27/52
CPCA61L27/08A61L27/227A61L27/50A61L27/52A61L27/54A61L2400/16A61L2430/20A61L2430/30A61L2430/32C08J3/075C08J3/24C08J2389/00C08K7/24C08K2201/011C08L89/00
Inventor 王琳王征王健李晓麟杨文张磊
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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