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Unsaturated fatty acid compound as well as preparation method and application thereof

A technology of unsaturated fatty acids and compounds, which is applied in the fields of unsaturated fatty acid compounds and their preparation and application, can solve the problem of strengthening the differentiation activity of nerve cells, which has not yet been discovered, and achieve the effect of strengthening the differentiation function of nerve cells

Active Publication Date: 2021-07-16
HKUST SHENZHEN RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have shown that the red-backed mountain hemp stalk has pharmacological activities such as anti-inflammation, anti-tumor and anti-alcoholic liver fibrosis, but there is no report on its ability to enhance nerve cell differentiation

Method used

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  • Unsaturated fatty acid compound as well as preparation method and application thereof
  • Unsaturated fatty acid compound as well as preparation method and application thereof
  • Unsaturated fatty acid compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Extraction, separation and structural identification of trewioidesine A

[0048] 1.1 Plant raw materials and sources: the dried roots of Alchorneatrewioides (Benth.) Muell. The root of the red-backed mountain hemp stem was collected from Heliao Town, Lianjiang City, Zhanjiang City, Guangdong Province in January 2020, and the samples were stored in the Chinese Medicine Research and Development Center of the Shenzhen Research Institute of the Hong Kong University of Science and Technology.

[0049] 1.2 Reagents for extraction and separation: Petroleum ether (Guangdong Guanghua Science and Technology Co., Ltd.), dichloromethane (Xilong Science Co., Ltd.), ethyl acetate (Tianjin Yongda Chemical Reagent Co., Ltd.), methanol (Xilong Science and Technology Co., Ltd.) for extraction and separation Co., Ltd.) etc. are of analytical grade; ultrapure water (Merck Direct-Q ultrapure water system).

[0050] 1.3 Chromatographic column and packing

[0051] Chromatographic...

Embodiment 2

[0060] Example 2: Activity detection of trewioidesine A inducing the expression of luciferase from the NF 200 promoter

[0061] The tested experiment consisted of the following steps:

[0062] (1) Culture PC12 cells (rat adrenal medulla pheochromoma differentiation cell line): at 37°C, 5% CO 2 And under saturated humidity conditions, the cells were placed in DMEM medium (containing 6% fetal bovine serum, 6% horse serum, 100 U / mL penicillin and 100 μm / mL streptomycin).

[0063] (2) The PC12 cells were divided into 8×10 4 / mL inoculated in a 24-well plate, and after 24 hours, the luciferase reporter gene vector pNF200-Luc containing Neurofilament200 encoding gene gene promoter was transfected into PC12 cells. After 4 hours, the medium was replaced with low serum medium (DMEM medium, 1% fetal bovine serum + 1% horse serum), and then trewioidesine A with different final concentrations was added.

[0064] (3) After 48 hours, the cell culture medium was removed and washed with PB...

Embodiment 3

[0067] Example 3: Test of trewioidesine A's effect on nerve cell differentiation

[0068] The tested experiment consisted of the following steps:

[0069] (1) Inoculate PC12 cells (2×10 4 cells / mL) into a 6-well plate, and after 24 hours, the medium was replaced with a low-serum medium (DMEM medium, 1% fetal calf serum+1% horse serum) to continue culturing for 24 hours.

[0070] (2) Subsequently, 10 μM trewioidesine A was added to treat for 48 hours, and the cell differentiation and neurite growth were observed. The treatment group with a final concentration of 20ng / mL NGF was used as a positive control, and the effect of combined treatment with a final concentration of 1ng / mL NGF was investigated at the same time.

[0071] (3) Take pictures with an optical microscope to analyze the growth of neurite, randomly select 5 fields of view in each well, and observe at least 100 cells in each field of view. Cells with one or more neurites longer than the cell body diameter were cl...

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Abstract

The invention relates to an unsaturated fatty acid compound as well as a preparation method and application thereof. According to the invention, the fatty acid compound is separated and identified from an ethyl acetate cold-soaked extract of dry root of alchornea rosea by adopting multiple chromatographic separation means and a spectrum-wave spectrum technology, and activity screening is performed through a PC12 cell (rat adrenal medullary pheochromoma differentiated cell strain) model to obtain the new unsaturated fatty acid compound with the function of enhancing nerve cell differentiation.

Description

technical field [0001] The present invention relates to the field of medicine, more particularly to a new unsaturated fatty acid compound isolated from the dry root of Alchornea trewioides (Benth.) Muell.Arg. The compound can promote luciferase activity of Neurofilament 200 promoter and PC12 cell differentiation, and has a significant synergistic effect with nerve growth factor (NGF), and provides a preparation method and application of the compound. Background technique [0002] Neurodegenerative diseases are characterized by the loss of neurons and / or their myelin sheaths, and are a class of complex diseases that seriously endanger human health. Alzheimer's disease (Alzheimer Disease, AD) is one of the most typical neurodegenerative diseases, characterized by memory and cognitive dysfunction, and is the most common type of Alzheimer's disease. Its pathogenesis is very complicated. There is no specific medicine yet. With the in-depth research on the pathogenesis of AD, a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/38C07D301/32A61P25/00A61K36/47
CPCC07D303/38C07D301/32A61P25/00A61K36/47
Inventor 詹华强董婷霞彭治添高雄王怀友杨金荣郭庆波
Owner HKUST SHENZHEN RES INST
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