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Application of 3beta-cinnamoyl diosgenin in induced differentiation of mesenchymal stem cells

A technology of diosgenin and cinnamoyl potato, which is applied in the field of stem cells and can solve problems such as no research report on diosgenin and its derivatives.

Inactive Publication Date: 2019-06-21
南京温博生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] So far, there are no research reports on the neural differentiation of diosgenin and its derivatives in bone marrow mesenchymal stem cells

Method used

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  • Application of 3beta-cinnamoyl diosgenin in induced differentiation of mesenchymal stem cells
  • Application of 3beta-cinnamoyl diosgenin in induced differentiation of mesenchymal stem cells
  • Application of 3beta-cinnamoyl diosgenin in induced differentiation of mesenchymal stem cells

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Embodiment Construction

[0022] The substantive content of the present invention will be described below in conjunction with specific embodiments, but the protection scope of the present invention cannot be limited thereto.

[0023] 1. Experimental materials

[0024] 4-week-old male SD rats of SPF grade, weighing 90-110 g, were provided by Qinglongshan Experimental Animal Center.

[0025] Fetal bovine serum, DMEM / F12 medium, and bFGF (basic fibroblast growth factor) were purchased from Gibco.

[0026] CD34, CD45, CD90, rabbit anti-Nestin, NSE, MAP-2, β-Actin and secondary antibodies were purchased from Abcam.

[0027] The purity of diosgenin and 3β-cinnamoyl diosgenin is not less than 95%.

[0028] 2. Experimental method

[0029] 1. Isolation, culture, purification and phenotypic identification of rat BMSCs

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Abstract

The invention discloses an application of 3beta-cinnamoyl diosgenin in induced differentiation of mesenchymal stem cells. Nestin is one of neural stem cell markers, expressed in early embryonic development; NSE is an acidic protein peculiar to neuron cells; and MAP-2 is a protein mainly existing in cell bodies and dendrites of neuronal cells, and plays an important role in synaptic growth. The Nestin, NSE and MAP-2 are marker proteins of neural cells, and the expression levels of the Nestin, NSE and MAP-2 can reflect the differentiation level of mesenchymal stem cells into neural cells. The differentiation ability of bone marrow mesenchymal stem cells to nerve cells is enhanced after being induced by 3beta-cinnamoyl diosgenin, and the bone marrow mesenchymal stem cells have similar effectsas positive drugs. Therefore, 3beta-cinnamoyl diosgenin can be used to induce differentiation of bone marrow mesenchymal stem cells into nerve cells in vitro, and can be made into commercial differentiation medium.

Description

technical field [0001] The invention belongs to the field of stem cells and relates to the induction and differentiation of stem cells, in particular to the application of 3β-cinnamoyl diosgenin in the induction and differentiation of mesenchymal stem cells. Background technique [0002] Bone marrow mesenchymal stem cells (BMSCs) are pluripotent stem cells derived from bone marrow, because they can differentiate into a variety of cardiomyocytes, osteoblasts, neuron-like cells, etc. Cells, with a wide range of sources and strong proliferation ability, are currently a hot spot in regenerative medicine research. In particular, as seed cells, it has an important application prospect in replacing neural stem cells with limited sources for the treatment of central nervous system injury and repair. [0003] Chemical induction is an important method to induce BMSCs to differentiate into neural-like cells in vitro. For the first time, Woodbury et al. induced BMSCs to differentiate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/079C12N5/0775
Inventor 王二赵艺婧其他发明人请求不公开姓名
Owner 南京温博生物科技有限公司
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