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Quality detection method of Eleutherine plicata Herb and extract thereof

A quality inspection method and extract technology, which are applied in the fields of plant raw materials, plant/algae/fungus/moss components, measuring devices, etc., can solve the problem of lack of shallot and its extract quality standards, and achieve quality assurance and clinical efficacy. , The effect of low detection components and strong operability

Inactive Publication Date: 2013-05-08
广州白云华南生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the Chinese Pharmacopoeia 2010 edition, there is no quality standard for shallots and their extracts

Method used

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  • Quality detection method of Eleutherine plicata Herb and extract thereof
  • Quality detection method of Eleutherine plicata Herb and extract thereof
  • Quality detection method of Eleutherine plicata Herb and extract thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 red onion medicinal material character and TLC identification

[0052] 【Properties】This product is oblong oval or long oval. Bulbs oblong, with thick scales, purple-red, without membranous coating. Roots tender, reddish brown. The leaves are curled, and the complete leaves are broadly lanceolate or strip-shaped after unfolding, 10-15cm long and 0.5-1.5cm wide, with apex acuminate and parallel longitudinal veins. Slight gas, light taste. The results are shown in the accompanying drawings of the figure 1 .

[0053] [Identification] (1) Take 1.5g of red onion medicinal powder, add 50ml of 60% ethanol solution, heat and reflux for extraction for 1 hour, filter, steam the filtrate until it has no alcohol smell, shake and extract with petroleum ether (30-60°C) for 3 Each time, 20ml each time, combined petroleum ether solution, water for later use, evaporated to dryness in petroleum ether solution in a water bath, and dissolved the residue with 2ml of methano...

Embodiment 2

[0055] Embodiment 2 shallot extract TLC identification

[0056] Get red onions, add 8 times the amount of 70% ethanol to extract twice, each time for 1 hour, combine the extracts, filter, recycle the ethanol from the filtrate, concentrate to a thick paste with a relative density of 1.30~1.40 (60°C), and vacuum dry ( 60~70℃, -0.08mpa), and the dry paste is crushed into fine powder.

[0057] The quality control methods described in this method include:

[0058] [Identification] (1) Take 1.5g of red onion extract, grind it finely, add 50ml of 60% ethanol solution, heat and reflux for 1 hour, filter, steam the filtrate until it has no alcohol smell, and shake it with petroleum ether (30-60°C) Extract 3 times, 20ml each time, combine the petroleum ether liquid, water liquid for later use, evaporate the petroleum ether liquid to dryness in a water bath, add 2ml of methanol to the residue to dissolve, and use it as the test solution. Take another 1.5 g of shallot medicinal material...

Embodiment 3

[0060] Embodiment 3 Determination of red onion medical material content

[0061] 1. Instruments and reagents

[0062] (1) Instrument: Shimadzu UV-2550 ultraviolet spectrophotometer.

[0063] (2) Reagents: Reagents are all analytically pure. Rutin reference substance (batch number: 10080-200707) was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0064] 2. Experimental conditions

[0065] (1) Preparation of reference substance solution

[0066] Accurately weigh 5 mg of rutin reference substance dried at 120°C to constant weight, put it in a 50ml measuring bottle, add an appropriate amount of methanol, dissolve it by ultrasonication, let it cool, add methanol to the mark, shake well, and obtain.

[0067] (2) Preparation of sample solution

[0068] Take about 1.5g of red onion medicinal powder, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 50ml of 60% ethanol solution precisely, seal it tightly, weigh it, heat an...

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Abstract

The invention discloses a quality detection method of Eleutherine plicata Herb and an extract of the Eleutherine plicata Herb. The method comprises character identification of the Eleutherine plicata Herb, thin-layer chromatography of the Eleutherine plicata Herb and the extract of the Eleutherine plicata Herb, and content detection of total flavones and total anthraquinones. The quality detection method comprises specific steps as follows: (1) the characters: illustrating the characters, the colors, the size and the smell of the Eleutherine plicata Herb; (2) the thin-layer chromatography: extracting different polar parts of the medicinal material and extract thereof, and expanding the polar parts on a silica gel plate by using different development systems; and (3) the content detection: detecting the content of the total flavones and the total anthraquinones in the Eleutherine plicata Herb and the extract of the Eleutherine plicata Herb. According to the method disclosed by the invention, based on ultraviolet spectrophotometry, rutin and rhein are respectively used as a reference substance, and the Eleutherine plicata Herb or the extract of the Eleutherine plicata Herb is used as a test sample to detect the content of the test sample. The method disclosed by the invention has the advantages that the method is convenient and quick, the pertinence of the detection method is powerful, the precision, the stability and the repeatability are good, and the accuracy is high. And the method is applicable to the quality control of medicinal materials or the extract of the medicinal materials.

Description

technical field [0001] The invention belongs to a quality detection method of medicines, in particular to a quality detection method of shallots and extracts thereof. Background technique [0002] Red onion, the whole herb of Eleutherine plicata Herb., a plant of the family Iridaceae, originates in the West Indies and is distributed in Guangxi, Yunnan and other places in my country. It is a folk medicinal plant. "Chinese Materia Medica" contains that it has the functions of clearing heat and cooling blood, promoting blood circulation and stimulating menstrual flow, reducing swelling and detoxification. Indications for hematemesis, hemoptysis, dysentery, abdominal pain due to amenorrhea, rheumatic arthralgia, bruises, boils and swollen toxins. According to folk herbal medicine, it can be used for menorrhagia, red metrorrhagia, epistaxis, gastrointestinal bleeding, traumatic bleeding, dysentery, rheumatic arthralgia, bruises and swelling, sores; Dai medicine uses bulbs for kn...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N21/33A61K36/88A61P7/04A61P15/00A61P29/00A61P31/04
Inventor 严超群王红星
Owner 广州白云华南生物科技有限公司
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