Production method for lipid through mixed culture of microbes

A mixed culture and microbial technology, applied in the biological field, can solve the problem of not promoting the growth of algae and oil accumulation, etc., and achieve the effects of increasing oil harvesting, efficient growth process, and improving accumulation effect.

Active Publication Date: 2014-07-30
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

The above-mentioned prior art is to control the growth balance of algae in the water body through bacteria, and has no effect of promoting the growth of algae and the accumulation of oil in algal cells

Method used

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  • Production method for lipid through mixed culture of microbes

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Embodiment Construction

[0019] The method for producing oil by mixed cultivation of microorganisms of the present invention specifically includes the following contents: a stirred bioreactor is used for facultative anaerobic bacterial seed liquid cultivation, an illuminated bioreactor is used for autotrophic microalgae seed liquid cultivation, and a stirred biological reaction The vessel includes facultative anaerobic bacteria and bacterial fermentation medium, and the illuminated bioreactor includes microalgae species and autotrophic medium. Before the mixed culture, the two microorganisms are separately subjected to expanded cell culture. After the expanded medium is qualified, the two microorganisms at different culture periods are mixed, and the mixed culture is continued. The organic carbon source in the mixed medium is used for the growth of facultative anaerobic bacteria, and the CO produced by the growth of facultative anaerobic bacteria 2 For the growth of microalgae. The mixed culture proces...

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Abstract

The invention discloses a production method for lipid through mixed culture of microbes. The method comprises the following steps: (1) culturing facultative anaerobic bacteria seed liquid and carrying out enlarged culture until the OD value of biomass is 5.0 to 15.0; (2) culturing autotrophic microalgae seed liquid and carrying out enlarged culture until the OD value of biomass is 2.0 to 10.0; and (3) mixing facultative anaerobic bacteria having undergone enlarged culture and autotrophic microalgae having undergone enlarged culture and carrying out mixed culture in an illuminated bioreactor. Compared to the prior art, the method provided by the invention has the advantages of increased harvest yield of autotrophic microalgae, an improved utilization rate of an inorganic carbon source (CO2), increased content of microbial lipid, a simplified culture apparatus and the like, and meanwhile, the method exerts small influence on the fermentation process of the facultative anaerobic bacteria, thereby realizing synchronous culture of the facultative anaerobic bacteria and the autotrophic microalgae.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method of using autotrophic oil-producing microalgae to obtain high cell yield and at the same time increase the oil content of microalgae cells. Background technique [0002] With the increasing shortage of oil resources, the rising oil prices, the increasingly prominent contradiction between oil supply and demand, and the more prominent environmental pollution problems, it is inevitable to develop renewable resources through multiple channels. Diesel from microbial grease has the properties of high energy density, low sulfur content, full combustion, good lubricity, etc. It also has the characteristics of renewable, easily biodegradable, safe storage and transportation, and good anti-knock performance. It can be used as a substitute for petrochemical energy. Product. [0003] Microalgae is one of the oil-containing microorganisms. Microalgae are rich in nutrients such as pro...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/20C12P7/64C12R1/01C12R1/07C12R1/145C12R1/225C12R1/22C12R1/89
Inventor 王领民金平李晓姝师文静张霖王崇辉高大成乔凯黎元生
Owner CHINA PETROLEUM & CHEM CORP
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