Traditional Chinese medicinal recipe for inhibiting vibrio anguillarum

A technology of Chinese herbal medicine and Vibrio eel is applied in the direction of pharmaceutical formulations, antibacterial drugs, medical preparations containing active ingredients, etc. The effect of drug resistance, no drug resistance, and simple production process

Inactive Publication Date: 2013-05-22
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term abuse of drugs or antibiotics will cause water environment pollution, which will lead to deterioration of the breeding environment, imbalance of ecological balance, and bacteria will develop drug resistance, and drug residues will pose a potential threat to consumers, while Chinese herbal medicines have no drug resistance and no residues , no environmental pollution, no drug-induced diseases and obvious antibacterial and bactericidal effects, more and more people's attention

Method used

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  • Traditional Chinese medicinal recipe for inhibiting vibrio anguillarum
  • Traditional Chinese medicinal recipe for inhibiting vibrio anguillarum
  • Traditional Chinese medicinal recipe for inhibiting vibrio anguillarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The invention is a Chinese herbal medicine formula for inhibiting Vibrio anguillarum, and the following 42 kinds of Chinese herbal medicines all have different degrees of antibacterial effects on Vibrio anguillarum.

[0011] First, Schisandra chinensis, pomegranate peel and mugwort leaves had the strongest antibacterial effect on Vibrio anguillarum, and the minimum inhibitory concentration (MIC) all reached 12.5 mg / mL. Secondly, aloe vera, alum, catechin, and herbaceous grass also have strong antibacterial effects on Vibrio anguillarum, and their MICs are all 25mg / mL. In addition, plants such as Burnet, lobelia, gonggong leaves, betel nut, papaya, Chinese tallow, moxibustion myrrh, pulsatilla, capillary, araceae, and skullcap also have certain antibacterial effects on Vibrio anguillarum, and their MICs are all 50mg / mL. Andrographis paniculata, Polygonum cuspidatum, Gentleman, Dandelion, Hops, Purslane, Forsythia, Gentiana, Atractylodes, Danpi, Morning Glory, Clove, Viol...

Embodiment 2

[0015] The invention is a Chinese herbal medicine formula for inhibiting Vibrio anguillarum. The Chinese herbal medicine is composed of the following raw materials and weight ratio: pomegranate peel, mugwort leaves, schisandra and aloe are mixed in a ratio of 1:4:1:4. The results showed that the formula has a significant antibacterial effect on Vibrio anguillarum, which is more obvious than the antibacterial effect of single herbal medicine and other combinations of Chinese herbal medicine compound formula, and its minimum inhibitory concentration MIC is 3.906mg / mL.

[0016] The minimum inhibitory concentration MIC of the Chinese herbal compound formula made by mixing pomegranate peel, mugwort leaves, schisandra and aloe at a ratio of 1:1:1:1 is 25mg / mL, and the ratio of pomegranate peel, mugwort, schisandra and aloe at a ratio of 4:1:4: The minimum inhibitory concentration MIC of the compound formula of Chinese herbal medicine mixed at a ratio of 8 is 26.563 mg / mL, which is no...

Embodiment 3

[0018] In vitro antibacterial test of Chinese herbal medicine on Vibrio anguillarum

[0019] 1. Selection of Chinese herbal medicine and preparation of liquid medicine

[0020] The 86 kinds of Chinese herbal medicines selected in this study were all purchased from Beijing Tongrentang Pharmacy in Ningbo City. The 86 kinds of Chinese herbal medicines are: schisandra, Galla gall, ebony, clematis, catechu, dijincao, purple diding, dry alum, prunella, goose does not eat, myrobalan meat, perilla leaves, leaking reed seeds, cassia seeds, Papaya, Radix Isatidis, Coptis chinensis, Senecio, Aloe Vera, Clove, Chinese tallow, Artemisia argyi, Polygonum cuspidatum slices, Crane lice, Gentiana, Snakeberry grass, Gonggong leaves, Forsythia, Myrrh, Rhubarb, Perrin, Torreya chinensis, Capillary, Phellodendron cortex, taro, andrographis paniculata, perilla, honeysuckle, purslane, astragalus, licorice, araceae, artemisia annua, lobelia, white peony, calamus, angelica, radish, aster, hedyotis di...

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Abstract

The invention discloses a traditional Chinese medicinal recipe for inhibiting vibrio anguillarum. The recipe is characterized by comprising at least one of fructus schisandrae chinensis, pericarpium granati, folium artemisiae argyi, aloe, aluminis usti, catechu, siegesbeckia pubescens mak, radix sanguisorbae, herba lobeliae chinensis, folium ilex, semen arecae, chaenomeles speciosa, sapium sebiferum, moxibustion myrrha, anemone chinensis, herba artemisiae scopariae, rhizoma arisaematis, radix scutellariae, andrographis paniculata, rhizoma polygoni cuspidate slice, fructus quisqualis, herba taraxaci, hops, herba portulacae, fructus forsythiae, gentiana scabra bunge, rhizoma atractylodis, cortex moutan radicis, semen pharbitidis, flos caryophyllata, herba violae, radix clematidis, solanum nigrum, galla chinensis, leguminosae, herba hedyotis, bupleurum chinense, tetradium ruticarpum, fructus mume, folium isatidis, plantago asiatica L and rhizoma curcumae, wherein a recipe which is formed by mixing the pericarpium granati, the folium artemisiae argyi, the fructus schisandrae chinensis and the aloe according to the mass ratio of 1:4:1:4 has an optimal antibacterial effect. The recipe has the advantages of naturality, no toxity and residues, no tolerance and low price.

Description

technical field [0001] The invention relates to a Chinese herbal medicine formula, in particular to a Chinese herbal medicine formula for inhibiting Vibrio anguillarum. Background technique [0002] Vibrio anguillarum ( Vibrio anguillarum ) is a Gram-negative opportunistic pathogenic bacteria with flagella, which can induce disease when aquaculture animals encounter adverse stimuli or injuries under adverse environmental conditions. Vibrio anguillarum can cause more than 50 species of marine and freshwater cultured fish and other aquatic animals worldwide to become ill. It is widespread and often occurs in temperate and sub-frigid regions. In 1999, my country's Lin Kebing and others reported on the harm of Vibrio in my country. There are many routes of infection of Vibrio anguillarum, mainly including skin, gills, lateral line and intestinal tract. After infection, it will cause systemic tissue lesions. Different fish and shrimps will have different symptoms after being i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9066A61K36/889A61K36/8884A61K36/86A61K36/81A61K36/754A61K36/748A61K36/739A61K36/736A61K36/732A61K36/716A61K36/71A61K36/704A61K36/68A61K36/634A61K36/61A61K36/57A61K36/539A61K36/48A61K36/39A61K36/185A61K35/64A61K33/06A61P31/04
Inventor 龚一富章丽张文青王何瑜童丽娟
Owner NINGBO UNIV
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