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Tissue culture and fast propagation method of South China Sea azalea

A technology for tissue culture and Rhododendron, applied in the field of plant cultivation, can solve the problems of difficult cutting, small seed quantity of Rhododendron in the South China Sea, and low emergence rate, and achieves the effects of solving protection and development, strong disease resistance and robust growth.

Inactive Publication Date: 2013-06-05
刘家迅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

South China Sea Rhododendron has small seeds, low emergence rate, and difficult cuttings. Therefore, it is necessary to find a new set of efficient and feasible propagation methods for propagation to meet market demand.

Method used

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  • Tissue culture and fast propagation method of South China Sea azalea
  • Tissue culture and fast propagation method of South China Sea azalea
  • Tissue culture and fast propagation method of South China Sea azalea

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1. Sterilization of explants: Collect annual shoots of Rhododendron nanhai, cut into 1 cm long bud stem segments, wash with soapy water, and then disinfect them in alcohol with a volume concentration of 75% in an ultra-clean workbench 20 seconds, sterilized in mercuric chloride solution with a mass concentration of 0.1% for 8 minutes, sterilized in a sodium hypochlorite solution with a volume concentration of 1.5% for 8 minutes, and then rinsed with sterile water for 3 times;

[0040] 2. Adventitious bud induction culture: Inoculate the explants sterilized in the above step 1 into the following induction medium in a sterile working environment: WPM+ZT2mg / L+IAA0.5mg / L+sugar 30g / L+agar 4g / L, PH5.5, cultured for 30 days under the conditions of light intensity of 1200LX, culture temperature of 25°C, and photoperiod of 8h / d, 65% of the explants were free from pollution, and clustered buds were induced. Cut off the bud cluster in a sterile environment, divide it into individu...

Embodiment 2

[0045] 1. Sterilization of explants: collect the annual shoots of Rhododendron chinensis, cut into 2 cm long bud stem segments, wash with soapy water, and then disinfect them in alcohol with a volume concentration of 75% in an ultra-clean workbench 30 seconds, sterilized in mercuric chloride solution with a mass concentration of 0.1% for 10 minutes, sterilized in a sodium hypochlorite solution with a volume concentration of 1.5% for 15 minutes, and then rinsed with sterile water for 3 times;

[0046] 2. Adventitious bud induction culture: inoculate the explants sterilized in the above step 1 into the following induction medium in a sterile working environment: WPM+ZT2.5mg / L+IAA0.8mg / L+sugar 30g / L+agar 5g / L, PH5.2, cultured for 30 days under the conditions of light intensity of 1500LX, culture temperature of 22°C, and photoperiod of 8h / d, 58% of the explants were free from pollution, and clustered buds were induced , cut off the bud cluster in a sterile environment, divide it ...

Embodiment 3

[0051] 1. Sterilization of explants: collect the annual shoots of Rhododendron nanhai, cut into 1 cm long bud stem segments, wash with soapy water, and then disinfect them in 70% alcohol in an ultra-clean workbench 40 seconds, sterilized in 0.15% mercuric chloride solution for 10 minutes, sterilized in 2% sodium hypochlorite solution for 15 minutes, and then rinsed with sterile water for 3 times;

[0052] 2. Adventitious bud induction culture: Inoculate the explants sterilized in the above step 1 into the following induction medium in a sterile working environment: WPM+ZT3mg / L+IAA1mg / L+sugar 30g / L+agar 7g / L L, PH5.5, cultured for 35 days under the conditions of light intensity of 1500LX, culture temperature of 20°C and photoperiod of 10h / d, 76% of the explants were free from pollution, and clustered buds were induced. Cut off the bud cluster in the environment, be divided into individual plants, and the individual plants are cut into stem segments with 2 nodes, and enter the n...

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Abstract

The invention discloses a tissue culture and fast propagation method of South China Sea azalea. A stem tip of the South China Sea azalea or an annual twig provided with an axillary bud stem section serves as an explant, is induced to generate an adventitious bud in an induction medium, a cluster bud which is needed is cultured in a multiplication medium, and the cluster bud is cut into individual plants, planted into a rooting medium, and directly planted into a nutritive bowl after rooting culture so that a South China Sea azalea seedling is obtained. The method is efficient and fast, can achieve the aim of improving the reproduction rate and reducing variation through hormonal regulation in each culture stage, and can be used for industrial and large-scale production of the South China Sea azalea.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of plants, in particular to a method for tissue culture and rapid propagation of rhododendron in the South China Sea, belonging to the technical field of plant cultivation. Background technique [0002] South China Sea Rhododendron belongs to Rhododendronaceae Rhododendron, Rhododendron genus, Rhododendron genus Rhododendron evergreen, shrub to small tree, 1-5 meters high, leathery leaves, flowering period 4-5 months, flower color purplish red, a unique species in my country, produced in Guangdong and Hainan and other places, is one of the few low-altitude heat-resistant evergreen rhododendrons, which can be widely used in landscaping in southern regions, and is also an excellent parent for cultivating heat-resistant evergreen rhododendrons. There are very few wild species. South China Sea Rhododendron has small seeds, low emergence rate, and difficult cuttings. Therefore, it is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘家迅
Owner 刘家迅
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