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Application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing gingivitis

A green olive and extract technology, which is applied in the field of plant extracts for preventing and treating gingivitis, can solve problems such as the inhibition of oral anaerobic bacteria by no green olive extracts.

Inactive Publication Date: 2013-06-05
LOGISTICS UNIV OF CAPF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there is no report on the inhibition of oral anaerobic bacteria by green olive extract

Method used

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  • Application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing gingivitis
  • Application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing gingivitis
  • Application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing gingivitis

Examples

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preparation example Construction

[0023] The preparation steps of the green olive extract are as follows: taking green olive branches, leaves or fruits as raw materials, carrying out reflux extraction with water or ethanol aqueous solution, filtering, concentrating the filtrate under reduced pressure to obtain a concentrate, and drying to obtain the green olive extract.

[0024] The preparation parameters of the green olive extracts of Examples 1-14 are shown in Table 1.

[0025] Table 1

[0026]

Embodiment 15

[0027] Example 15 Test of green olive extract inhibiting actinomycetes, streptococcus sanguinis and gingival capnocytophagy that induce gingivitis in vitro

[0028] Actinomyces viscosus (Actinomyces viscosus) ATCC15987 was purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0029] Streptococcus sanguinis (Streptococcus sanguinis) ATCC10556 was purchased from Beijing Beina Chuanglian Biotechnology Research Institute

[0030] Capnocytophaga gingivalis ATCC33624, purchased from Beijing Beina Chuanglian Biotechnology Research Institute

[0031] Test method: Take out Actinomyces viscosus (ATCC15987), Streptococcus sanguinis ATCC10556, and Capnocytophaga gingivalis ATCC33624 from the -80°C refrigerator respectively, resuscitate them routinely, and inoculate them on BHI agar Culture medium, anaerobic culture at 37°C (80% N 2 , 10%H 2 , 10% CO 2 ) 48h, pick a single colony and replant it in BHI liquid medium, and culture it anaerobically at 37°C for 48h. A...

Embodiment 16

[0035] The influence of the green olive extract of embodiment 16 different concentrations on the amount of dental plaque formed by Actinomyces viscosus

[0036] Test method: In this experiment, the plant extract prepared in Example 4 was used to carry out the following tests. The blank control group was Actinomyces viscosus suspension (200 μL per well). The negative control group was a polyethylene 96-well plate containing absolute ethanol (10 μL per well) containing Actinomyces viscosus suspension (190 μL per well). In the experimental group, 1 / 4MIC, 1 / 2MIC, MIC, 2MIC green olive extracts (10 μL per well) were added to polyethylene 96-well plates containing Actinomyces viscosus suspension (190 μL per well). In triplicate, the same concentration level was repeated 4 times. 37 ℃ anaerobic culture for 48h. Suck out the bacterial suspension in the wells with a micropipette, gently wash the wells 3 times with PBS to remove planktonic bacteria, add fresh BHI liquid medium (200 μ...

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Abstract

The invention discloses application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing gingivitis. Experiments prove that green olive extract can obviously suppress actinomyces viscosus, streptococcus sanguis and capnocytophaga gingivalis which cause gingivitis, the actinomyces viscosus, the streptococcus sanguis and the capnocytophaga gingivalis are prevented from forming dental plaque, so that the green olive extract has an obvious preventing and curing function on gingivitis. The green olive extract can be prepared into various medicines or food or daily necessities, and thus gingivitis is prevented and cured conveniently.

Description

technical field [0001] The invention relates to a plant extract for preventing and treating gingivitis, in particular to the application of green olive plant extract in the fields of food, medicine and oral preparations for preventing and treating gingivitis. Background technique [0002] The plant extract involved in the present invention is from the plant extract of green olive which is abundant in my country. The green olive tree Canarium album (Lour.) Raeusch. is a kind of economic fruit tree planted in parts of southern my country. Its mature and dry fruit is oval and blue in color. It is also known as green fruit, green seed, white olive, green olive, etc. The effective active ingredients are olive polyphenols, which have the functions of clearing heat, relieving throat, promoting body fluid, detoxification, immune regulation, lowering blood pressure, lowering blood fat, and scavenging free radicals. It is used to treat sore throat, cough, polydipsia, fish and crab pois...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/32A61K8/97A61P1/02A23L1/29A23G3/48A23G4/06A61Q11/00A61K135/00A61K127/00A61K131/00A23L33/00
Inventor 刘岱琳赵艳敏陈虹延慧君马卫俊高颖
Owner LOGISTICS UNIV OF CAPF
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