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Antituberculous CTL (Cytotoxic T Lymphocyte) epitope peptide with drug-resistant related efflux protein source for tuberculosis and application of epitope peptide

A technology for efflux proteins and epitope peptides, which can be used in the fields of peptides, antibacterial drugs, and pharmaceutical formulations, and can solve the problem of few reports on membrane proteins

Active Publication Date: 2013-06-05
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CD8 currently studied + CTL protein epitopes are mainly concentrated on tuberculosis-specific secreted proteins, such as ESAT-6, CFP-10, CFP-21, MPT64, Ag85 complexes, etc., while for membrane proteins, especially the drug efflux protein CD8+CTL Epitopes are rarely reported

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  • Antituberculous CTL (Cytotoxic T Lymphocyte) epitope peptide with drug-resistant related efflux protein source for tuberculosis and application of epitope peptide
  • Antituberculous CTL (Cytotoxic T Lymphocyte) epitope peptide with drug-resistant related efflux protein source for tuberculosis and application of epitope peptide
  • Antituberculous CTL (Cytotoxic T Lymphocyte) epitope peptide with drug-resistant related efflux protein source for tuberculosis and application of epitope peptide

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Embodiment Construction

[0013] The anti-tuberculosis CTL epitope peptide derived from the tuberculosis drug resistance-related efflux protein of the present invention is a nonapeptide, and the amino acid sequence of the nonapeptide is:

[0014] P5: Tyr-Leu-Gly-Gly-Thr-Thr-Gly-Pro-Val (YLGGTTGPV) molecular weight: 864.6 (theoretical value: 863.44)

[0015] Or P6: Tyr-Ile-Val-Gly-Phe-Cys-Leu-Leu-Val (YIVGFCLLV) Molecular weight: 1026.7 (theoretical value: 1025.86)

[0016] or P7: Thr-Leu-Thr-Trp-Leu-Phe-Ala-Phe-Val (TLTWLFAFV) molecular weight: 1097.7 (theoretical value: 1097.32)

[0017] or P8: Gly-Leu-Val-Ala-Gly-Leu-Ser-Ala-Val (GLVAGLSAV) molecular weight: 786.7 (theoretical value: 786.7)

[0018] Or P9: Ala-Leu-Gly-Met-Leu-Ile-Ala-Gly-Leu (ALGMLIAGL) molecular weight: 858.7 (theoretical value: 857.81)

[0019] or P10: Met-Leu-Ile-Ala-Gly-Leu-Pro-Cys-Leu (MLIAGLPCL) molecular weight: 930.6 (theoretical value: 930.24)

[0020] or P11: Leu-Leu-Cys-Ala-Ile-Phe-Ala-Glu-Val (LLCAIFAEV) molecular weig...

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Abstract

The invention discloses an antituberculous CTL (Cytotoxic T Lymphocyte) epitope peptide with a drug-resistant related efflux protein source for tuberculosis. The antituberculous CTL epitope peptide is nonapeptide, wherein the amino acid sequence of the nonapeptide is P9: ALGML IAGL. Predicative analysis is carried out on HLA-A*0210 restrictive CTL epitope of drug-resistant related protein antigen for tuberculosis by adopting immune-informatics means and SYFPEITH1, BIMAS and NetCTL1.2 databases according to the primary structure of the antigen, so that the epitope peptide is obtained by screening; and the identified nonapeptide has not been reported in any document. An in-virto ELISPOT (Enzyme-Linked Immunospot Assay) is adopted to identify the epitope peptide; and the identification result provides a theoretical basis for developing tuberculosis vaccine based on drug-resistant related protein antigen and provides more information for designing multi-epitope peptide vaccine for tuberculosi based on the mixed T cell epitope.

Description

[0001] The patent application of the present invention is a patent application number: 201110138646.2, application date: May 26, 2011, and a divisional application titled "Anti-tuberculosis CTL epitope peptide derived from efflux protein related to tuberculosis drug resistance and its application". technical field [0002] The present invention relates to a therapeutic polypeptide vaccine for tuberculosis, in particular to an anti-tuberculosis CTL epitope peptide with therapeutic activity screened out by using the tuberculosis self-resistance-related antigen, and also relates to the application of the peptide in the preparation of a therapeutic polypeptide vaccine for tuberculosis . Background technique [0003] The increasing number of drug-resistant Mycobacterium tuberculosis is an important reason for the resurgence of tuberculosis in recent years. The drug resistance mechanism of Mycobacterium tuberculosis can be divided into natural drug resistance mechanism and acquire...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K39/00A61P31/06
Inventor 祁元明陈飞高艳锋朱宇皇吴亚红陈艳平韩艳林
Owner ZHENGZHOU UNIV