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Sulfoxide alkaloid compound as well as preparation method and application for same

A technology of alkaloids and compounds is applied in the field of microorganisms to achieve the effect of improving the development prospects of new anti-cancer drugs and new antibacterial drugs

Inactive Publication Date: 2013-06-12
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no sulfoxides have been found from marine microbes

Method used

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  • Sulfoxide alkaloid compound as well as preparation method and application for same
  • Sulfoxide alkaloid compound as well as preparation method and application for same
  • Sulfoxide alkaloid compound as well as preparation method and application for same

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Inoculate Streptomyces marititus into tryptone soybean broth liquid medium, culture at 28°C and 200rpm for 48 hours to obtain Streptomyces marititus seed culture; the seed liquid is inoculated into a 500ml Erlenmeyer flask containing 150ml fermentation medium at 10% inoculum A total of 50 bottles were cultured at 28°C and 200 rpm for 432 hours to obtain 7L of marine Streptomyces fermentation broth. The fermentation broth was centrifuged at 6000 r / min for 10 min to obtain the fermentation broth supernatant and mycelium. The supernatant was mixed with ethyl acetate in a ratio of 1:2, extracted three times repeatedly, and evaporated to dryness using a rotary evaporator to obtain an ethyl acetate extract of the supernatant; the mycelium used methanol / acetone (1:1, v:v) After wall-breaking extraction for 12 hours, centrifuge at 6000 r / min for 10 minutes and take the supernatant to obtain the methanol / acetone mycelium extract. Use a rotary evaporator to remove the methanol / ace...

Embodiment 2

[0032] Use an inoculating loop to pick a single colony of Staphylococcus aureus in the plate, inoculate it in 4ml of LB medium, and incubate at 37°C and 200rpm for 4-6 hours to make the final concentration of about 1~2×10 8 CFU / ml; Take 13 sterile test tubes and arrange them in a row. Except for the first tube with 1.6ml LB medium, add 1ml of LB medium to each tube, and add Xinghaixinine 1280 μg / ml 0.4 Mix well, and then pipette 1ml to the second tube. After mixing, pipette 1ml to the third tube, and then dilute to the 11th tube by successive times, and then aspirate 1ml from the 11th tube and discard it. The 12th tube contains no Growth control of the drug. At this time, the drug concentration of each tube was 256, 128, 64, 32, 16, 8, 4, 2, 1, 0.5, 0.25 μg / ml. Then add 1ml each of the above-prepared inoculum to each tube, so that the final bacterial concentration of each tube is about 5×10 5 CFU / ml. The drug concentration of the first to the 11th tube is 128, 64, 32, 16, 8, 4...

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Abstract

The invention discloses a sulfoxide alkaloid compound as well as a preparation method and an application for the same, and belongs to the field of microbial technology. The sulfoxide alkaloid compound is obtained by inoculating marine streptomyces into a tryptone soy broth liquid culture medium, culturing for 48-72 hours at a temperature of 28-30 DEG C and with a speed of 180-200 rpm, inoculating the marine streptomyces into a fermentation culture medium according to 10% of inoculum size, culturing for 432-456 hours at a temperature of 28-30 DEG C and with a speed of 180-200 rpm, and separating and purifying the fermentation solution. The compound has a remarkable inhibition effect on various bacteria, in particular, on staphylococcus aureus, and a certain anti-tumour activity for human breast cancer cells MCF-7, human liver cancer cells SMMC-7721, human acute myelomatosis cells U937 and small cell lung cancer cells NCI-H1688 simultaneously. Therefore, the compound has a good prospect for developing new anti-cancer medicines and new antibacterial medicines.

Description

Technical field [0001] The invention relates to a sulfoxide alkaloid compound and its preparation and application, belonging to the technical field of microorganisms. Background technique [0002] With the widespread clinical application of antibiotics, the continuous emergence of clinically resistant pathogens and the emergence of SARS, avian influenza and super bacteria in recent years", there is an urgent need to develop new antibiotic drugs. Many active substances produced by actinomycetes have For medicine and plant protection purposes, it has been widely used as antibacterial, antifungal and antitumor drugs. Of the tens of thousands of biologically active substances derived from microorganisms that have been discovered, about 70% are synthesized by actinomycetes. However, with the over-exploitation of terrestrial actinomycetes, the probability of discovering novel biologically active compounds is decreasing year by year. Marine microorganisms live in special environments su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D519/00A61K31/439A61P35/00A61P11/00A61P1/16A61P35/02A61P31/04C12P17/18C12R1/465
Inventor 赵心清矫文策白凤武
Owner DALIAN UNIV OF TECH
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