Method for on-line synthesizing mannose-6-acetate by lipase catalysis
A technology of mannose and lipase, which is applied in the field of online controllable and selective synthesis of mannose-6-acetate catalyzed by lipase, which can solve the problems of long reaction time, low conversion rate and low selectivity, and reduce usage , short reaction time, high conversion and reaction selectivity
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Embodiment 1
[0026] Example 1: Synthesis of Mannose-6-Acetate
[0027]
[0028] device reference figure 1 : Mannose (0.4mmol) was dissolved in 10mL tert-amyl alcohol: DMSO=4:1 (v / v) mixed solvent, vinyl acetate (8.4mmol) was dissolved in 10mL tert-amyl alcohol, and then packed in 10mL Ready to use in the syringe. 0.87g of lipase Lipozyme TLIM was evenly filled in the reaction channel of the microfluidic channel reactor, and driven by the PHD2000 syringe pump, the two reaction solutions were separated at 10.4 μL min -1 The flow rate enters the reaction channel through the "Y" joint for reaction, and the temperature of the reactor is controlled at 52°C by a water bath thermostat. The reaction solution flows continuously in the reaction channel for 30 minutes, and the reaction results are tracked and detected by thin-layer chromatography (TLC).
[0029] The reaction solution was collected online by the product collector, the solvent was distilled off under reduced pressure, and the colum...
Embodiment 2-5
[0033] Change the temperature of the microfluidic channel reactor, others are the same as in Example 1, and the reaction results are as shown in Table 1:
[0034] Table 1: Effect of Temperature on Reaction
[0035]
[0036] The results in Table 1 show that when the flow rate is 10.4 μL min -1 , when the reaction time is 30min, the conversion rate increases obviously with the increase of the reaction temperature. When the reaction temperature reaches 52°C, the conversion rate and selectivity of the reaction are the best. The decrease in enzyme activity leads to a decrease in the conversion rate and selectivity of the reaction, so the optimal reaction temperature of mannose acetate in the microfluidic microchannel reactor in the present invention is 52°C.
Embodiment 6-11
[0038] Change the substrate molar ratio of vinyl acetate and mannose in the microfluidic microchannel reactor to be 17: 1 (Example 6), 18: 1 (Example 7), 19: 1 (Example 8), 20: 1 (Example 9), 22:1 (Example 10), 23:1 (Example 11), and others are the same as Example 1. The results are shown in Table 2.
[0039] Table 2: Effect of Mannose to Vinyl Acetate Substrate Ratio on Reaction
[0040] Example Vinyl acetate:mannose Conversion rates[%] selectivity [%] 6 17∶1 55 100 7 18∶1 75 100 8 19∶1 88 100 9 20∶1 95 100 1 21∶1 98 100
[0041] 10 22∶1 97 99 11 23∶1 96 98
[0042] The results in Table 2 show that as the reactant vinyl acetate increases, the conversion rate of the reaction also increases. When the substrate ratio is 21:1, the conversion rate and selectivity of the reaction are optimal, and mannose has basically been quantified Complete conve...
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