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Ultrasonic microwave extraction method of spartina anglica total alkaloids and use of antibacterial activity of the spartina anglica total alkaloids

A technology of total alkaloids and Spartina, applied in the fields of application, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc. Effectively implement and obtain satisfactory results, etc., to achieve the effect of mild operating conditions and high yield

Inactive Publication Date: 2013-07-10
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the differences between different plants in geographical growth environment and family and genus species, there are differences in the active ingredient composition and content of different plants, and the actual ultrasonic operation conditions are different. According to the above-mentioned documents and invention patents, it is very difficult to extract the total alkaloids of Spartina. Difficult to implement effectively and achieve satisfactory results

Method used

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  • Ultrasonic microwave extraction method of spartina anglica total alkaloids and use of antibacterial activity of the spartina anglica total alkaloids
  • Ultrasonic microwave extraction method of spartina anglica total alkaloids and use of antibacterial activity of the spartina anglica total alkaloids
  • Ultrasonic microwave extraction method of spartina anglica total alkaloids and use of antibacterial activity of the spartina anglica total alkaloids

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Experimental program
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Effect test

Embodiment 1

[0018] Embodiment 1: Extraction of total alkaloids of Spartina

[0019] The collected fresh Spartina is pretreated: washed with tap water, dried in vacuum, crushed and sieved. Take 40g of 40-mesh powder, dissolve it in 400mL of 70% ethanol according to the ratio of material to liquid (w / v) of 1:10, and extract it by ultrasonic wave for 50min. The extract was obtained by suction filtration, and the extract was evaporated at 35°C to the extract, and the extract was dissolved with citric acid to adjust the pH value to 2.5, and the anhydrous Na 2 CO 3 Adjust the pH value to 10.5 to produce crude alkaloid precipitation. After centrifugation, the supernatant was discarded to obtain a precipitate, which was collected and dried, and weighed to obtain 0.5286 g of crude alkaloid. It can be calculated that the extraction rate is 1.32%.

Embodiment 2

[0020] Embodiment 2: Extraction of total alkaloids of Spartina

[0021] The collected fresh Spartina is pretreated: washed with tap water, dried in vacuum, crushed and sieved. Take 40g of 80-mesh powder, dissolve it in 400mL of 80% ethanol according to the ratio of material to liquid (w / v) 1:30, and extract it by ultrasonic wave for 60min. The extract was obtained by suction filtration, and the extract was evaporated at 40°C to the extract, and the extract was dissolved with citric acid to adjust the pH to 3, and the anhydrous Na 2 CO 3 Adjust the pH value to 10 to produce crude alkaloid precipitation. After centrifugation, the supernatant was discarded to obtain a precipitate, which was collected and dried, and weighed to obtain 0.4729 g of crude alkaloid. It can be calculated that the extraction rate is 1.18%.

Embodiment 3

[0022] Embodiment 3: Extraction of total alkaloids of Spartina

[0023] The collected fresh Spartina is pretreated: washed with tap water, dried in vacuum, crushed and sieved. Take 40g of 20-mesh powder, dissolve it in 400mL of 60% ethanol according to the ratio of material to liquid (w / v) 1:20, and extract it by ultrasonic wave for 40min. The extract was obtained by suction filtration, the extract was evaporated at 30°C to the extract, and the extract was dissolved with citric acid to adjust the pH value to 1, and the anhydrous Na 2 CO 3 Adjust the pH value to 11 to produce crude alkaloid precipitation. After centrifugation, the supernatant was discarded to obtain a precipitate, which was collected and dried, and weighed to obtain 0.4963 g of crude alkaloid. It can be calculated that the extraction rate is 1.24%.

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Abstract

The invention relates to an ultrasonic microwave extraction method of spartina anglica total alkaloids and a use of an antibacterial activity of the spartina anglica total alkaloids obtained by the ultrasonic microwave extraction method. The ultrasonic microwave extraction method comprises the following steps of drying spartina anglica, crushing the dried spartina anglica into spartina anglica powder, carrying out screening, adding the screened spartina anglica powder into ethanol, carrying out ultrasonic extraction, carrying out pumping filtration to obtain an extract liquid, carrying out rotary evaporation to obtain extract, dissolving the extract in citric acid to adjust a pH value to less than 7, adjusting a pH value to more than 7 by anhydrous Na2CO3 so that spartina anglica total alkaloids are precipitated, carrying out centrifugation, removing a supernatant, collecting precipitates, and drying the collected precipitates to obtain the spartina anglica total alkaloids. The spartina anglica total alkaloids have a certain effect of inhibiting growth of escherichia coli, pseudomonas aeruginosa and bacillus amyloliquefaciens.

Description

technical field [0001] The invention relates to a preparation method and application of an extract of Spartina, in particular to a method for extracting total Spartina alkaloids and the application of Spartina Spartina total alkaloids prepared by the method in antibacterial aspects. Background technique [0002] Spartina belongs to the Poaceae Spartina genus, which is a perennial wet herbaceous perennial plant. Spartina contains a variety of biologically active substances, such as alkaloids, amino acids, vitamins, etc. The content of crude protein is relatively high, and it is a plant with relatively complete nutritional components. Alkaloids are a large class of basic nitrogen-containing organic compounds that widely exist in nature. Some alkaloids have anti-tumor, anti-inflammatory, antibacterial, anti-platelet aggregation, anti-arrhythmia and anti-hypertensive effects on cardiovascular diseases. , so that it has great application potential in health, medicine, etc., and ...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61P31/04A23L1/30
CPCY02A50/30
Inventor 许伟邵荣丁建飞颜秀花
Owner YANCHENG INST OF TECH
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