Supercharge Your Innovation With Domain-Expert AI Agents!

Determination method of liquid phytase stability

A technology of liquid phytase and determination method, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of inactivation of enzyme activity, not considering the important influence of shock factors, and decreasing clarity.

Inactive Publication Date: 2013-07-10
北京昕大洋科技发展有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Existing methods for evaluating the stability of liquid phytase, the first is to judge its stability qualitatively only by observing whether there is precipitation, and the second is to not consider the shock factors in the actual transportation, storage and use of its clarity and enzyme activity. important influence
It can be seen from the following table 1 that some liquid phytases are severely inactivated by shaking relative to standing still; the absorbance increases and the clarity decreases severely

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Determination method of liquid phytase stability
  • Determination method of liquid phytase stability
  • Determination method of liquid phytase stability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Oscillation test at room temperature at 25°C

[0053] Liquid phytase in transportation, storage, and use of absorption spray, etc., all have large shakes and shocks to the liquid enzyme, which is easy to cause flocculation of the liquid enzyme. The experiment was carried out at room temperature of 25°C using a thermostatic oscillator for different periods of time (oscillation intensity was 200 revolutions per minute) to observe the changes in enzyme activity and absorbance at 546nm (h is hour and d is day). The test liquid phytase comes from 4 products A, B, C, and D collected from the market, and the manufacturers are respectively Xin Dayang, Xinhua Yang, Kang Dian, and Tian Tian Xiang.

[0054] The changes in enzyme activity and retention rate after shaking are shown in Table 2. From the data in the table, it can be seen that the enzyme activity stability of sample A is the best, followed by sample B, D is poor, and sample C has the worst stability.

[0055...

Embodiment 2

[0061] Example 2 Oscillation test at high temperature at 37°C

[0062] In this test, a constant temperature shaker was used to keep the temperature at 37°C and shake continuously at a speed of 200 rpm. In addition, four liquid phytase samples A, B, C, and D were collected from the market, and the manufacturers were Xin Dayang and Xinhua respectively. Yang, Kang Dien, Tian Tianxiang. Repeat the above experiment, observe the change of enzyme activity and the change of absorbance at 546nm (h is hour, d is day).

[0063] Table 4 High temperature 37 ℃ shaking enzyme activity test data (unit U / g)

[0064]

[0065]

[0066] It can be seen from Table 4 that liquid phytase sample A still retains a retention rate of more than 90% after 7 days of enzyme activity at 37°C, while the enzyme activity of other samples is lost to 0 after one day of high temperature shaking at 37°C. It can be seen that the enzyme activity of sample A is the most stable, followed by B, D is poor, and C s...

Embodiment 3

[0070] Example 3 High Temperature 45°C Oscillation Test

[0071] In order to quickly detect or compare the stability of different liquid phytases, use a water bath constant temperature oscillator to shake the liquid phytase in a water bath at 45°C for 1 hour at a speed of 200 rpm, and compare the conditions before and after shaking at 546nm. The stability of the liquid phytase can be judged by the change of enzyme activity and absorbance. The four liquid phytases A, B, C, and D used come from Xin Dayang, VTR, Challenge, and Xinhuayang respectively.

[0072] Table 6 High temperature 45 ℃ shaking enzyme activity test data (unit U / g)

[0073]

[0074] Table 6 shows that after shaking at 45°C for 1 hour, the enzyme activity of sample A is the most stable, followed by sample B, D is worse, and sample C is the worst.

[0075] Table 7 High temperature 45 ℃ shock test absorbance change data

[0076]

[0077]

[0078] It can be seen from Table 7 that after shaking at 45°C f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for determining liquid phytase stability. The method comprises the steps that: (1) liquid phytase is subjected to an oscillation treatment for 10min-120h under a temperature of 6-60 DEG C; (2) during the treatment process in the step (1), at least one time point is selected for determining light absorbance and enzyme activities of the liquid phytase before and after oscillation; (3) clarity stability is evaluated according to the change in light absorbance obtained in the step (2); and (3) enzyme activity stability is evaluated according to the change in enzyme activities obtained in the step (2). According to the invention, a method for effectively and quantitatively comparing stabilities of various liquid phytase is provided. Specially, a method for determining stabilities of various liquid phytase through comparing the changes in light absorbance and enzyme activities of different liquid phytase before and after oscillation is provided by the invention.

Description

technical field [0001] The invention relates to a method for measuring the stability of liquid phytase. Background technique [0002] Phosphorus is one of the essential mineral elements for animals, and it is very important to provide sufficient phosphorus in animal diets. Although plant feed materials contain a large amount of phosphorus, 60-80% of them are in the form of phytate phosphorus, and monogastric animals and aquatic animals lack the enzymes required to decompose phytate phosphorus, and the utilization rate of phosphorus It is only 0-30%, so it is necessary to add a large amount of inorganic phosphorus in the feed to supplement the deficiency of phosphorus. This will cause a large increase in the cost of the feed, and at the same time the phytate phosphorus in the feed cannot be utilized by the animals and is excreted in large quantities out of the animals, causing serious pollution to the soil and water. [0003] Phytase is a feed enzyme preparation that can hy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/31
Inventor 苏俊兵魏秀莲郭宝林
Owner 北京昕大洋科技发展有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com