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Soil heavy metal hexavalent chromium biological degrading agent and production method thereof

A biodegradant, heavy metal technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reducing soil fertility, the effect is not significant, and the implementation is complicated, so as to improve the soil and benefit human health. , Improve the effect of soil water and fertilizer retention and air permeability

Active Publication Date: 2013-07-17
FOSHAN JINKUIZI PLANT NUTRIMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These treatment technologies are not widely used due to factors such as complex implementation, high cost, easy reduction of soil fertility, easy reactivation, long cycle and insignificant effect.

Method used

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  • Soil heavy metal hexavalent chromium biological degrading agent and production method thereof
  • Soil heavy metal hexavalent chromium biological degrading agent and production method thereof
  • Soil heavy metal hexavalent chromium biological degrading agent and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The main components of a soil heavy metal hexavalent chromium biodegradation agent are WH2 strains, WH5 strains, WH8 strains, WH20 strains, medical stones, oyster shells, and active organic materials.

[0033] Among the above microorganisms, the strain WH2 is Bacillus subtilis; the strain WH5 is Brevibacterium sulfurum; the strain WH8 is Streptomyces fradiae; the strain WH20 is Pseudomonas aeruginosa bacteria (Pseudomonas Aeruginosa).

[0034] The ratio of bacteria contained in the soil heavy metal hexavalent chromium biodegradant is (weight percent):

[0035] (1) WH2 strain: 10%; (2) WH5 strain: 13%;

[0036] (3) WH8 strain: 40%; (4) WH20 strain: 37%.

[0037] The manufacture method of this soil heavy metal hexavalent chromium biodegradation agent comprises bacterial strain cultivation and preparation, and its process step is:

[0038] (1) The above-mentioned WH2 strains, WH5 strains, WH8 strains, and WH20 strains are uniformly mixed according to the above weight pe...

Embodiment 2

[0049] The proportioning and parameter of each composition of the soil heavy metal hexavalent chromium biodegradation agent of the present embodiment are different from example 1:

[0050] 1. The proportion of bacteria contained in the soil heavy metal hexavalent chromium biodegradant is (weight percent):

[0051] (1) WH2 strain: 26%; (2) WH5 strain: 50%;

[0052] (3) WH8 strain: 8%; (4) WH20 strain: 16%.

[0053] 2. The ratio (percentage by weight) of the secondary strain culture medium is: active organic material 35%, rice bran 45%, soybean powder 17%, milk powder 1.5%, sugar 0.5%, and the rest water.

[0054] 3. The temperature of the secondary strain culture medium is 45°C, the pH value is 6.5, and the culture time is 10 days, and the number of viable bacteria can reach 1.5 billion / g.

[0055] 4. Mix medical stones, oyster shells, active organic materials and active furfur of WH flora according to the following weight percentage formula, ferment at room temperature for m...

Embodiment 3

[0063] The proportioning of each composition of soil heavy metal hexavalent chromium biodegradation agent of the present embodiment and each parameter and example 1 difference are:

[0064] 1. The proportion of bacteria contained in the soil heavy metal hexavalent chromium biodegradant is (weight percent):

[0065] (1) WH2 strain: 45%; (2) WH5 strain: 30%;

[0066] (3) WH8 strain: 17%; (4) WH20 strain: 8%.

[0067] 2. The ratio (percentage by weight) of the secondary strain culture medium is: active organic material 45%, rice bran 35%, soybean powder 15%, milk powder 2.5%, sugar 1.5%, and the rest water.

[0068] 3. The temperature of the medium for secondary strains is 30°C, the pH value is 7.5, the culture time is 15 days, and the number of viable bacteria can reach 500 million / g.

[0069] 4. Mix medical stones, oyster shells, active organic materials and active furfur of WH flora according to the following weight percentage formula, ferment at room temperature for more th...

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Abstract

The invention discloses a soil heavy metal hexavalent chromium biological degrading agent and a production method thereof. The soil heavy metal hexavalent chromium biological degrading agent comprises the following main components: a strain WH2, a strain WH5, a strain WH8, a strain WH20, medical stone, shuck and active organic material. The production method of the soil heavy metal hexavalent chromium biological degrading agent comprises the following processing steps of: mixing the strains WH2, WH5, WH8 and WH20 in proportion to form a WH colony, carrying out secondary strain culture on the WH colony by virtue of a WH colony secondary culture medium, finally mixing the medical stone, shuck and active organic material with active bran bacteria of the WH colony in a formula, fermenting for more than 12 days at normal temperature, smashing, and packaging to obtain the soil heavy metal hexavalent chromium biological degrading agent. An effect is obvious when the biological degrading agent is utilized for degrading heavy metal hexavalent chromium in soil.

Description

technical field [0001] The invention relates to the technical field of soil heavy metal hexavalent chromium biodegradation, in particular to a soil heavy metal hexavalent chromium biodegradation agent and a manufacturing method thereof. Background technique [0002] With the advancement of urbanization in our country, chemical pollution has become a major source of pollution. Benzene, phenol, phosphorus organic pollution and heavy metal pollution such as cadmium, arsenic, lead, chromium, mercury are serious. While polluting air and water, they have also become long-standing "cancers" in the soil. Industry insiders pointed out that whether heavy metals pollute water bodies or pollute the atmosphere, they will eventually return to the soil and cause soil pollution. According to the Ministry of Land and Resources (reported by Nanfang Daily on April 1, 2011), more than 10% of the country's cultivated land has been polluted by heavy metals, about 150 million mu. [0003] There ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/125C12R1/13C12R1/54C12R1/385
Inventor 何飚黄恩雄何定国何定荣
Owner FOSHAN JINKUIZI PLANT NUTRIMENT CO LTD
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