Unlock instant, AI-driven research and patent intelligence for your innovation.

Electrochemical drug sensor used for detecting acetaminophen, and preparation method thereof

A paracetamol and sensor technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve sensitive detection and wide application prospects

Inactive Publication Date: 2013-07-24
SHANGHAI UNIV
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current drug standard uses the permanent stop titration method to determine the content of acetaminophen. This method is complicated to operate and has interference from other components, which makes it difficult to observe the determination end point and affects the results of content determination.
At present, the methods for detecting acetaminophen mainly include gas chromatography, ultraviolet spectrophotometry, thin layer chromatography, etc., but the above methods are still relatively time-consuming

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical drug sensor used for detecting acetaminophen, and preparation method thereof
  • Electrochemical drug sensor used for detecting acetaminophen, and preparation method thereof
  • Electrochemical drug sensor used for detecting acetaminophen, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of carbon powder electrode for modifying DNA fibers

[0022] Take a one-milliliter syringe, remove both ends with a knife, smooth it on sandpaper, clean it with ethanol and water, and sonicate for 20 min. Clean and dry the plastic tube after ultrasonication, and put it aside for use. Flatten a section of the push rod of the syringe and wash it for later use. Weigh the DNA fiber, graphite powder, and paraffin oil respectively according to a certain mass ratio, first pour the powder solid into a 50 mL centrifuge tube, put them on a shaker and shake them well, then put them and the oil into Grind thoroughly in a pre-washed mortar. Fill a part of the uniformly ground carbon paste into a section of the plastic tube, press the carbon paste tightly with the push rod of the syringe, insert a copper wire, and then fill the remaining carbon paste into the plastic tube and press it firmly together with the wire. Tape the copper wire and push rod to preven...

Embodiment 2

[0023] Example 2: Process for detecting acetaminophen

[0024] Submerge the working electrode carbon paste electrode in the drug sensor in 10mL of the buffer solution containing the analyte, control the potential from 0V to 0.8V and scan once with cyclic voltammetry at a scan rate of 50 mM / s to check the sample Electronic activity in . The liquid to be tested is 10 mM phosphate buffer containing a certain concentration of paracetamol and 0.15 M NaCl.

[0025] like figure 1 As shown, when the carbon powder electrode is not modified with DNA fibers, the electrode adsorbs less acetaminophen, and the sample shows an oxidation peak of about 0.65V; while when the carbon paste electrode is modified with DNA fibers, the electrode adsorbs The acetaminophen in the sample increased, the sample oxidation peak became larger, and the peak potential shifted negatively, which indicated that the DNA fiber increased the electron transfer, and the carbon powder electrode containing DNA fiber ...

Embodiment 3

[0026] Example 3: Optimization of Acetaminophen Detection Conditions

[0027] In order to detect acetaminophen more sensitively, we optimized the detection conditions and studied the influence of pH value on the peak current of the analyte.

[0028] The prepared carbon paste electrode modified with DNA was soaked in the buffer solution of different pH containing the same concentration of acetaminophen, and the electrochemical detection was carried out by cyclic voltammetry. like figure 2 , when the pH is 7.4, the peak current corresponding to the detected substance is the largest, which indicates that the optimum pH value is 7.4.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an electrochemical drug sensor used for detecting acetaminophen, and a preparation method thereof. The sensor is a three-electrode-system sensor with a counter electrode of a platinum electrode, a reference electrode of a silver / silver chloride electrode, and a working electrode of a carbon powder electrode. The sensor is characterized in that DNA fiber is modified on the carbon paste electrode. According to the sensor, with the sensitivity of DNA molecules and good adhesion of the DNA molecules with molecules to be detected, and with high sensitivity and convenience of electrochemical detection, rapid and sensitive detection is carried out upon acetaminophen. Theoretically, the detection can also be carried out upon other molecules with high bonding force with bases. The sensor and the method have wide application prospect.

Description

technical field [0001] The invention relates to a novel electrochemical drug sensor and a preparation method thereof, in particular to an electrochemical drug sensor for detecting acetaminophen and a preparation method thereof. Background of the invention [0002] Acetaminophen is a main ingredient in Ganmaoling Granules, molecular formula C 8 h 9 NO 2 , usually white crystalline powder, is the active ingredient of antipyretic and analgesic drugs, has antipyretic and analgesic effects, and is used for headache, fever, nasal congestion, runny nose, sore throat, etc. caused by colds. In my country, the production and consumption of preparations containing acetaminophen are huge, and doctors and patients do not know much about the ingredients of different medicines, which may easily lead to repeated or excessive use, and poisoning due to accidental ingestion. Studies have shown that acetaminophen in excess or under certain special circumstances (such as drinking, starvation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 陈红霞刘峰
Owner SHANGHAI UNIV