Preparation method and application of mouse syncytiotrophoblast microvillous membrane

Abstract

The invention discloses a preparation method and an application of a mouse syncytiotrophoblast microvillous membrane. The preparation method comprises the following steps of: based on mouse placenta as a raw material, separating and enriching the mouse syncytiotrophoblast microvillous membrane by mechanical separation and improved differential centrifugation methods; detecting the concentration by protein concentration quantification and identifying and quantitatively marking by polypeptide antigens of tissues. The invention further discloses the application of the mouse syncytiotrophoblast microvillous membrane prepared by the preparation method in building a mouse model with hypertensive disorder complicating pregnancy. The mouse syncytiotrophoblast microvillous membrane prepared by the method disclosed by the invention is large in yield and high in concentration, and is highly similar to human syncytiotrophoblast microvillous membrane in shape and protein marker. The mouse syncytiotrophoblast microvillous membrane can be directly used to research occurrence and development mechanism of hypertensive disorder complicating pregnancy such as preeclampsia and eclampsia as well as medical base and clinical research for developing antagonistic medicines, and can be further used to prepare an animal model of hypertensive disorder complicating pregnancy.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and application of a mouse syncytiotrophoblast microchorion. Background technique [0002] The syncytiotrophoblast microchorion is a membrane-bound vesicle-like structure formed when the syncytiotrophoblast is apoptotic or activated, and is currently thought to be associated with a variety of pregnancy complications. The syncytiotrophoblast microchorion shed into the maternal blood circulation can act on peripheral blood vessels, leading to dysfunction of vascular endothelial cells, and at the same time affect the maternal immune system by regulating the release of inflammatory factors in the body and regulating cellular immunity. [0003] There has been a lot of evidence suggesting the relationship between syncytiotrophoblast microchorion and hypertensive disorders during pregnancy such as preeclampsia and eclampsia, but based on the ethical ...

AI Technical Summary

Problems solved by technology

[0003] There has been a lot of evidence suggesting the relationship between syncytiotrophoblast microchorion and hypertensive disorders during pregnancy such as preeclampsia and eclampsia, but based on the ethical requirements of medical research and the special nature of the pregnancy process, clinical trials have not yet been conducted Specific role and mechanism of syncytiotrophoblast microchorion in human diseases

In addition, animal experiments and in vivo studies were not possible due to the absence of suitable animal models targeting syncytiotrophoblast microchorions

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