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A method of verifying allelopathy of plants by using chlorophyll fluorescence

A technology of chlorophyll fluorescence and allelopathy, applied in the field of plant physiology and ecology, can solve the problems of inaccurate judgment and low verification efficiency, and achieve the effect of clear response

Active Publication Date: 2015-06-17
江苏蕨美植物科技有限公司
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Problems solved by technology

The existing method of using chlorophyll fluorescence to verify plant allelopathy mainly has defects such as low verification efficiency and inaccurate judgment

Method used

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  • A method of verifying allelopathy of plants by using chlorophyll fluorescence
  • A method of verifying allelopathy of plants by using chlorophyll fluorescence
  • A method of verifying allelopathy of plants by using chlorophyll fluorescence

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Experimental program
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Embodiment

[0009] A method for verifying plant allelopathy with chlorophyll fluorescence, comprising the steps of:

[0010] 1. Data collection and analysis of plant chlorophyll fluorescence;

[0011] 2. Verification of allelopathic effects on changes in plant chlorophyll.

[0012] The step 1, data collection and analysis of allelopathic plant chlorophyll fluorescence; specifically includes,

[0013] 1. Experimental materials:

[0014] Basic modulated chlorophyll fluorescence instrument (Junior-Pam, Heinz Walz GmbH, D91090, Effeltrich); 15 disposable plastic petri dishes; 20-day pre-cultured Limegrass gametophytes;

[0015] 2. Experimental treatment design:

[0016] 1) Take the 20-day-pre-cultured Limegrass gametophytes, and put them into 10mL disposable plastic culture of root exudates with concentration gradients of 100%, 50%, 25%, 12.5% ​​and 0%. In a dish, keep 100 gametophytes per dish, and each group has three parallel groups with a mass concentration gradient; the experiment ti...

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Abstract

The invention is a method of verifying allelopathy of plants by using chlorophyll fluorescence, and the method comprises the following steps: 1, collection and analysis of plant chlorophyll fluorescence data; and 2, verification of allelopathy on plant chlorophyll content change. The method is advantageous in that the response of allelopathy on plant physiological changes can be determined quickly, efficiently and purposefully, especially the influence on plant photosynthesis.

Description

technical field [0001] The invention relates to a method for verifying plant allelopathy by using chlorophyll fluorescence, and belongs to the field of plant physiology and ecology. technical background [0002] Allelopathy in English is "Allelopathy", which comes from the Greek " Allelon (mutual)" and " Pathos (damage, hindrance)". The theory of plant allelopathy was first proposed by German scientist H. Molish in 1937. He defined allelopathy as: the interaction of biochemical substances between all types of plants (including microorganisms) However, Molish believes that this interaction includes both harmful and beneficial aspects. In the mid-1970s, E.L. Rice defined plant allelopathy as defined by H.Molish’s definition and further research on plant allelopathy. Plants (including microorganisms) produce direct or indirect harmful effects on other plants (including microorganisms) through chemical substances released into the environment. The allelopathic substances in R...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 张开梅沈羽方炎明
Owner 江苏蕨美植物科技有限公司
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