Canine parvovirus nucleic acid rapid detection kit
A canine parvovirus and detection kit technology, applied in the biological field, can solve the problems of long time, high operator requirements, high price, etc., and achieve the effect of simplifying processing conditions and time, shortening reaction time, and low instrument requirements
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[0050] Example 1, rapid detection of canine parvovirus nucleic acid
[0051] Canine parvovirus nucleic acid rapid detection kit was used. The canine parvovirus nucleic acid rapid detection kit comprises:
[0052] 1. Lysis buffer 500μL;
[0053]
[0054] 2. Diluent rehydrate buffer 1.8ml;
[0055] Each 80ml diluent contains:
[0056]
[0057] 3. 4ml of freeze-dried enzyme tube containing primers; the components in the freeze-dried enzyme tube containing primers are preserved after freeze-drying;
[0058]
[0059]
[0060] Wherein, the sequence of the primer pair is as follows:
[0061] CGP00740: 5'FAM-CAATTGGAGGTAAAGCAGGAATTAAC-3'
[0062] CGP00719: 5'Biotin-GGTGCATTTACATGAAGTCTTGGTTTTAAGTC-3'
[0063] 4. Detection solution;
[0064]
[0065] 5. Test strips: colloidal gold test strips that specifically recognize FAM and Biotin.
[0066] The steps for using the canine parvovirus nucleic acid rapid detection kit are as follows.
[0067] 1. Take an appropri...
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