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A rapid NMR method for the detection of foodborne pathogens based on indirect enrichment of paramagnetic nano-Ni probes

A kind of food-borne pathogenic bacteria, paramagnetic nanotechnology, applied in the direction of measuring devices, analysis by nuclear magnetic resonance, instruments, etc.

Active Publication Date: 2016-04-13
苏州辅测技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature reporting this method at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Test food samples for the presence of the harmful pathogen Listeria monocytogenes.

[0022] 1. Preparation of nano-Ni immunoprobe: Antibody 1 is rabbit anti-IgG monoclonal antibody against Listeria monocytogenes, antibody 2 is goat anti-rabbit IgG against Listeria monocytogenes. Buy nano-Ni materials from the market, such as Beijing Deke Daojin Technology Co., Ltd. or Shanghai Chaowei Nano Materials Co., Ltd., 20nm, purity 99.9%.

[0023] Silica-coated nano-Ni: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-Ni and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85°C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the p...

example 2

[0030] Determination of food samples for the presence of harmful pathogenic bacteria Escherichia coli O157:H7.

[0031] 1. Nano-Ni immunoprobe preparation: 1 anti-rabbit anti-IgG monoclonal antibody of O157:H7, and 2 anti-goat anti-rabbit IgG of O157:H7, which can be monoclonal antibody or polyclonal antibody. Ni nanoparticles can be purchased from the market (such as Chaowei Nanomaterials Company in Shanghai).

[0032] Silica-coated nano-Ni: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-Ni and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85°C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed i...

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PUM

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Abstract

A fast detection method of NMR food -based pathogenic bacteria based on the indirectly enrichment of Magnetic nano -Ni probe, which belongs to the fast detection technology field of food safety pathogenic bacteria.The invention depends on the nuclear magnetic resonance testing method that can be used in the food liquid sample in the food liquid sample, uses 1 anti -capture target bacteria, and uses 1 antibody, that is, 2 anti -packaged cosmetic nano Ni probesTo enrich and separate, the effects of nano -NI's smooth magnetic characteristics on the relaxation time of the nuclear magnetic resonance attenuation signal to detect whether the sample contains target bacteria.Different specific correspondences are: Magnetic nano-ni probe, showing a linear relationship under certain conditions, that is, the nano Ni content is large, the spin-spin-relaxation time of the sample or the time value of the spinning crystal relaxation time, the moreSmall, can quantitatively detect target bacteria in a certain range.This method can be used for fast testing of harmful pathogenic bacteria in food samples, which can be used as a rapid screening of a large number of samples to be checked.

Description

technical field [0001] The invention relates to a rapid detection method for pathogenic bacteria, in particular to an NMR rapid detection method for food-borne pathogenic bacteria based on the indirect enrichment of paramagnetic nano-Ni probes. Background technique [0002] Basic principle: Monoclonal antibodies or antigen molecules are combined with enzyme molecules through covalent bonds. This combination will not change the immunological characteristics and biological activities of monoclonal antibodies, antigens and enzymes. Specific monoclonal antibodies will only interact with specificity. antigen binding. Ni material has ferromagnetism, and paramagnetic properties will appear when the particle diameter is small enough to a certain extent. That is, there is no magnetism when there is no external magnetic field, but it shows certain magnetism when there is an external magnetic field, which can be used for magnetic separation. At the same time, the influence of paramag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08G01N33/577
Inventor 张锦胜唐群赖卫华
Owner 苏州辅测技术服务有限公司