Penicillium griseofulvum, antibacterial active compound generated thereby and application

A technology of Penicillium grisea and antibacterial activity, applied in the direction of microorganisms, antibacterial drugs, organic active ingredients, etc., can solve the problems of decreased efficacy of antibiotics, increased bacterial resistance, and ineffectiveness, achieving remarkable effects, obvious antibacterial activity, The process is simple and easy to control

Active Publication Date: 2013-08-21
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the widespread use of antibiotics, bacterial resistance has also increased year by year, resulting in reduced efficacy or even ineffectiveness of some antibiotics

Method used

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  • Penicillium griseofulvum, antibacterial active compound generated thereby and application
  • Penicillium griseofulvum, antibacterial active compound generated thereby and application
  • Penicillium griseofulvum, antibacterial active compound generated thereby and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The fermentation of embodiment 1 Penicillium grisea CPCC 400528 and the preparation of antibacterial active compound

[0027] Penicillium griseofulvum (Penicillium griseofulvum) CPCC 400528 of the present invention is isolated from a soil sample near Kanas Lake in Xinjiang, and has been preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC NO.5752.

[0028] A. Medium formula: (slant medium formula)

[0029]

[0030]

[0031] B. Fermentation medium formula:

[0032]

[0033] Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, pack into test tubes, sterilize at 121°C for 30 minutes, and prepare the slant. The preserved Penicillium grisea CPCC 400528 strain was transferred to the slant medium and cultured at 28°C for 5 days. Pick the mycelium block and inoculate it in the liquid fermentation medium, and cultivate it...

Embodiment 2

[0042] The active test of embodiment 2 antibacterial active compound (isopatulin)

[0043] I. Compound antibacterial activity test: measure the in vitro antibacterial activity of the active compound prepared in Example 1 with agar dilution method.

[0044] 1. Strains to be tested:

[0045] G as shown in Table 1 + Standard strains of Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium and their drug-resistant strains; Mycobacterium tuberculosis and G - Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii standard strains and their resistant strains, Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, Morgan Standard strains of Morganella, Prufidenia regiti, Proteus vulgaris, Proteus mirabilis, Salmonella typhi and Citrobacter freundii.

[0046] 2. Preparation of test samples and medium:

[0047] (1) The medium uses Mueller-Hinton (MH) or 7H11 agar medium, prepared according to conv...

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Abstract

The invention provides a?Penicillium griseofulvum?CPCC400528, the preservation number of which is CGMCC NO.5752. The Penicillium griseofulvum CPCC400528 can be fermented to generate an antibacterial active compound with a molecular formula of C7H6O4 and a structure shown as formula (I). The compound has a good growth inhibition effect on a variety of Gram positive pathogenic bacteria and Gram negative pathogenic bacteria. With the increase of concentration, the inhibition effect is gradually enhanced. Also, the compound has a good inhibition effect on a plurality of drug resistant strains. The Penicillium griseofulvum strain provided in the invention is easy to ferment and culture and has a high yield. The preparation process of the active compound is simple and is easy to control. The prepared compound has obvious antibacterial activity, lays the foundation for research and development of new antibacterial drugs, and has very broad market prospects.

Description

technical field [0001] The invention belongs to the field of microbiology and pharmacy, and in particular relates to a strain of Penicillium grisea, an antibacterial active compound produced therefrom and application thereof. Background technique [0002] The emergence of natural antibacterial drugs and synthetic antibacterial drugs has made it possible to effectively treat various bacterial infections, and has made outstanding contributions to the protection of human health. However, with the widespread use of antibiotics, bacterial drug resistance has also increased year by year, resulting in reduced efficacy or even ineffectiveness of some antibiotics. Some infectious diseases (such as tuberculosis, etc.) that have been controlled in the past have become popular again; some non-pathogenic bacteria have become conditional pathogens, such as Proteus, Pseudomonas aeruginosa, etc.; Let the medical community worry about the arrival of the "post-antibiotic era". Therefore, it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18C07D493/04A61K31/365A61P31/04C12R1/80
CPCY02A50/30
Inventor 余利岩王莉宁张志明张涛游雪甫胡辛欣刘红宇魏玉珍
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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