Fluorescence microscopy imaging system and method for monitoring neural network

A neural network and microscopic imaging technology, applied in the field of imaging systems, can solve the problems of long scanning time, inability to detect for a long time, and inability to achieve fast imaging of neural samples, and achieve the effect of short time

Active Publication Date: 2013-09-04
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problems of long scanning time, inability to detect for a long time, and the inability to realize rapid imaging of multicolor fluorescently l

Method used

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  • Fluorescence microscopy imaging system and method for monitoring neural network
  • Fluorescence microscopy imaging system and method for monitoring neural network
  • Fluorescence microscopy imaging system and method for monitoring neural network

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Embodiment 1

[0033] An embodiment of the present invention provides a fluorescence microscopy imaging system for monitoring neural networks, see figure 1 , the system includes: a light source 1 , a filter 2 , a microscope 3 , a photodetector 4 , and a control device 5 .

[0034] Wherein, the filter 2 is used to adjust the wavelength and light passing time of the light passing through the filter 2 under the control of the control device 5 . The photodetector 4 is used for wide-field imaging under the control of the control device 5 . The filter 2 , the microscope 3 and the photodetector 4 are sequentially located on the light path of the light source 1 . The control device 5 is electrically connected to the filter 2 and the photodetector 4 respectively. figure 1 Middle arrows indicate light propagation paths, and solid lines indicate electrical connections.

[0035] Specifically, the microscope 3 includes an objective lens and a stage for carrying a nerve sample marked with a fluorescent...

Embodiment 2

[0045] An embodiment of the present invention provides a fluorescent imaging method for monitoring neural networks, which is suitable for monitoring neural samples marked with fluorescent dyes. The fluorescent dyes labeled with neural samples may include one or more types. This embodiment will use a The present invention will be described by taking a fluorescent dye (first fluorescent dye) as an example. In this embodiment, the first fluorescent dye is the calcium fluorescent dye Oregon Green488BAPTA-2, AM, and the central wavelength of the excitation of Oregon Green488BAPTA-2, AM is 496 nm. see figure 2 , the method includes:

[0046] Step 201: During the first time period, adjust the wavelength range of the light that can pass through the filter to the first wavelength range, so as to obtain light with a wavelength in the first wavelength range, and use a light with a wavelength in the first wavelength range The neural sample is illuminated with light while widefield imag...

Embodiment 3

[0057] An embodiment of the present invention provides a fluorescent imaging method for monitoring neural networks, which is suitable for monitoring neural samples marked with fluorescent dyes. The fluorescent dyes labeled with neural samples may include one or more types. This embodiment will use two The present invention will be described by taking fluorescent dyes (first fluorescent dye and second fluorescent dye) as examples. In this embodiment, the first fluorescent dye is calcium fluorescent dye Oregon Green488BAPTA-2, AM, which excites Oregon Green488BAPTA-2, and the wavelength corresponding to the center frequency of AM is 496nm, and the second fluorescent dye is calcium fluorescent dye Oregon Green488Rhod-2, AM, excited Oregon Green488Rhod-2, the center wavelength of AM is 552nm. see image 3 , the method includes:

[0058] Step 301: During the first time period, adjust the wavelength range of the light that can pass through the filter to the first wavelength range,...

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Abstract

The invention discloses a fluorescence microscopy imaging system for monitoring a neural network, and belongs to the imaging system field. The system comprises a light source and a microscope which comprises an objective lens and a stage that is used for bearing a nerve sample marked with fluorescence dyes. The system also comprises: a control device; a filter for adjusting the wavelength and the light-transmitting time of light transmitting through the filter under the control of the control device; and a photoelectric detector for wide-field imaging under the control of the control device. The filter, the microscope and the photoelectric detector are arranged on an optical path of the light source in sequence, and the control device is electrically connected with the filter and the photoelectric detector respectively. The fluorescence microscopy imaging system spends a short time on detecting the whole nerve sample, meets requirements of research on the neural network, can detect for a long time, and also can realize fast switch when the nerve sample marked by multicolor fluorescence carries out imaging of different dyes.

Description

technical field [0001] The invention relates to the field of imaging systems, in particular to a fluorescent microscopic imaging system and method for monitoring neural networks. Background technique [0002] The nervous system is the highest center for regulating and controlling life activities, and neurons are the basic units that constitute the structure and function of the nervous system. The basic function of a neuron is to receive a stimulus and integrate the excitement, then transmit it to another neuron in the form of an action potential. Each neuron is interconnected with other neurons to form a network-like structure. Many advanced functions of the brain, such as cognitive formation, memory storage and restoration, etc., are performed by specific neural networks, so the study of neural networks is of great significance for exploring the unknown brain. [0003] At present, the commonly used method for studying neural networks is fluorescence imaging. In this metho...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 吴钰祥李超英
Owner JIANGHAN UNIVERSITY
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