Method for separating and purifying three flavonoid glycosides from stems and leaves of trichosanthes kirilowii

A technology for separation and purification of flavonoid glycosides, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve problems such as waste disposal, waste of resources, and insufficient utilization of stems and leaves, and achieve purity and Improve efficiency, prolong service life, and save reagents
CN103288903AInactive Publication Date: 2013-09-11LIAOCHENG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LIAOCHENG UNIV
Publication Date
2013-09-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for separating and purifying three flavonoid glycoside type components from stems and leaves of trichosanthes kirilowii. The stems and the leaves of the trichosanthes kirilowii are taken as raw materials, and the method comprises the following steps: (1) preparing a crude extract of the stems and the leaves of the trichosanthes kirilowii; (2) extracting; (3) performing semi-preparation type high performance liquid chromatography separation and purification: performing separation and purification on an ethyl acetate part by semi-preparation type high performance liquid chromatography, wherein a mobile phase is methanol-water, and three high-purity flavonoid glycoside type components, namely luteolin-7-O-beta-glucoside, apigenin-7-O-beta-glucoside and chrysoeriol-7-O-beta-glucoside are respectively obtained. The process flow is green and environment-friendly, has no serious harm to an environment and is low in comprehensive cost.
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Description

technical field

[0001] The invention belongs to the field of chemical industry, and in particular relates to a method of separating and purifying three kinds of flavonoid glycosides (luteolin-7-O-β-glucoside, apigenin-7-O-β-glucoside and cotyledon Ellisin-7-O-β-glucoside) method. Background technique

[0002] Cucurbitaceae Trichosanthes genus Trichosanthes ( Trichosanthes kirilowii Marim.) and Bilateral Trichosanthes ( Trichosanthes rosthorinii Harms) is the original plant of the Trichosanthes medicinal material stipulated in the Chinese Pharmacopoeia, and its fruit, peel, seeds and tubers are used as Trichosanthes, Trichosanthes skin, Trichosanthes seeds and trichosanthes respectively. The stems and leaves of Trichosanthes chinensis are sour, cold in nature, non-toxic, and return to the liver and spleen meridians. They have the effect of clearing away heat and relieving summer heat. Ascites-type cancer cells in the dead body have anti-cancer effects.

[0003] There a...

Claims

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