Unlock instant, AI-driven research and patent intelligence for your innovation.

Synthetic method of rare earth polysaccharide complex with antibacterial activity

A technology of antibacterial activity and synthesis method, which is applied in the field of synthesis of rare earth polysaccharide complexes, and can solve problems such as human health hazards and environmental pollution

Inactive Publication Date: 2016-06-15
QINGDAO AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, chemical pesticides are mainly used for resisting pests and diseases, weeding and promoting the growth of crops. However, the environmental pollution and harm to human health caused by the long-term use of these chemical pesticides have attracted increasing attention.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synthetic method of rare earth polysaccharide complex with antibacterial activity
  • Synthetic method of rare earth polysaccharide complex with antibacterial activity
  • Synthetic method of rare earth polysaccharide complex with antibacterial activity

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] Embodiment 1 has the synthetic method of the rare earth polysaccharide complex of antibacterial activity, comprises the following steps:

[0023] 1. Extraction of Ganoderma lucidum polysaccharides: The strain MP-01 of Ganoderma lucidum is inserted into the culture medium to obtain liquid strains, and then cultured in a fermenter, the supernatant is concentrated and then alcohol-precipitated, and finally the precipitate is centrifuged, washed, and dried Dried Ganoderma lucidum crude polysaccharide.

[0024] 2. Weigh 0.8g Ganoderma lucidum polysaccharide and disperse it in 30mL of isopropanol with a concentration of 0.03g / mL under constant stirring. After stirring at room temperature for 6h, add 40mL of 60% (m / V) sodium hydroxide solution, at 85℃ Reflux in water bath for 1.5h, then add 60mL of 60% (m / V) chloroacetic acid solution within 0.5h, and react at constant temperature at 65°C for 4h under constant stirring; or dissolve 2g of polysaccharide in 250mL of NaOH solutio...

Embodiment 4

[0044] Example 4 The bacteriostatic activity experiment of the rare earth polysaccharide complex prepared by the synthesis method of the rare earth polysaccharide complex with bacteriostatic activity of the present invention

[0045] The mycelial growth rate method is used, that is, the test agent is mixed with the medium, and the growth rate of the colony on the medium is used to measure the toxicity of the compound. Generally, it is mostly used for those fungi that do not produce spores or have a small amount of spores and dense mycelia. , to test the effect of Yb-carboxymethyl Ganoderma lucidum polysaccharide-complex on apple rot disease fungus (Valsamali Miyabeet Yamada), cotton wilt disease fungus (Fusarium oxysporum f.sp. ) and cabbage black spot fungus (Alternaria brassicae) five kinds of plant pathogenic bacteria in vitro antibacterial activity.

[0046] Five gradient concentrations of Yb-carboxymethyl ganoderma lucidum polysaccharide-complex solutions were prepared wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a synthesis method of a rare earth polysaccharide complex with antibacterial activity. The synthesis method comprises the following steps of: dispersing polysaccharides in isopropanol while stirring, adding a sodium hydroxide solution, performing reflux for 1.5h in a water bath at the temperature of 85 DEG C, and then adding a chloroacetic acid solution; performing constant-temperature reaction for 4h at the temperature of 65 DEG C while stirring continuously; cooling to room temperature after the end of reaction; performing rotary evaporation to remove the isopropanol, adjusting the pH of a reaction system to 7.00, performing suction filtration, then performing dialysis with distilled water for 3 days, and performing dialysis with ultra-pure water for 1 day; performing rotary evaporation, concentration and freeze-drying on a dialysate to get white flocculent carboxymethylated polysaccharides; weighing the carboxymethylated polysaccharides and dissolving in deionized water; dropwise adding a rare earth solution into the carboxymethylated polysaccharides, and adjusting the pH till neutral, namely pH=7.00 and the solution just produces a flocculent precipitate; and performing stirring reaction for 24h. After the end of the reaction, filtration is performed, and suction filtration, rotary evaporation, concentration and free-drying are performed on a filtrate to get a complex solid. The generated rare earth polysaccharide complex has the double actions of inducing plant immune response by the polysaccharides and directly inhibiting and killing pathogen cells by rare earth.

Description

technical field [0001] The invention relates to a method for synthesizing a rare earth polysaccharide complex with antibacterial activity. Background technique [0002] At present, chemical pesticides are mainly used for resisting pests and diseases, weeding and promoting the growth of crops. However, the environmental pollution and harm to human health caused by the long-term use of these chemical pesticides have attracted increasing attention. Recently, a wide range of raw materials for production, biopesticides that are safe to non-target organisms, have low toxicity and side effects, and have good environmental compatibility have become a new trend in the development of the global pesticide industry. Biopesticides mainly refer to the direct use of biologically produced active substances or living organisms as pesticides, as well as artificially synthesized pesticides with the same structure as natural compounds. Biopesticides are pesticides that are synthesized by extra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A01P3/00
Inventor 王进平孙晓波张翠
Owner QINGDAO AGRI UNIV