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A method for improving rice grain shape and grain weight

A grain type and rice technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of linkage burden, loss of good traits, etc., and achieve the effect of improving appearance quality and grain weight

Inactive Publication Date: 2016-06-15
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when a certain fragment is introduced in breeding to improve a certain trait, there will be loss of good traits due to linkage redundancy or pleiotropic effects.

Method used

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  • A method for improving rice grain shape and grain weight
  • A method for improving rice grain shape and grain weight
  • A method for improving rice grain shape and grain weight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Construction of near-isogenic lines

[0040] 1.1: Genetic effect analysis of the three grain type QTLs of the target

[0041] In the implementation process of the present invention, three grain type QTLs discovered by the National Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University were used: qGW2a controlling grain width on the second chromosome, qGL3 controlling grain length on the third chromosome and the fifth chromosome The qGS5 that controls the grain width on the top, the specific position on the rice genome is roughly as follows image 3 As shown (see Ma Dapeng, 2005; Lietal.2011). Among them, qGW2a is the main effect locus controlling grain width, which can explain 66.7% of the population grain width variation; qGL3 is the main effect QTL controlling grain length, which can explain 59.8% of the population grain length variation; qGS5 can explain the population 36.2% of the variation in grain width (Table 1). In the study...

Embodiment 2

[0050] Example 2: Preparation of Molecular Markers Closely Linked to QTLs and Identification of Marker Genotypes

[0051] 2.1 Preparation of molecular markers closely linked to QTL

[0052] Information such as the sequence and the position of the SSR mark used in the present invention all come from Gramene website database ( http: / / www.gramene.org / ). Also according to the genome sequence of the japonica rice variety Nipponbare published online ( http: / / rgp.dna.affrc.go.jp) and the genome sequence of indica rice variety 93-11 (indica rice variety 93-11 is a public database resource) (see: http: / / rise.genomics.org.cn / ) as a comparison to find and design polymorphic markers . The principle of marker design is: there is a 5-10 bp deletion or insertion between the genome sequence of the above-mentioned japonica rice variety Nipponbare and the genome sequence of the indica rice variety 93-11, and the PCR fragment is about 200-400 bp. Based on the QTL mapping results found by ...

Embodiment 3

[0058] Embodiment 3 The present invention is applied in rice breeding

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Abstract

The invention belongs to the technical field of paddy rice molecule auxiliary breeding, and particularly relates to a method for obtaining materials of different grain sizes and different weights by utilizing polymerization grain type QTL. The method adopts a breeding process of once crossbreeding, three times of backcross and once selfing, so as to lead the target grain type QTL into a paddy rice variety to-be-improved, and built a plurality of near-isogenic lines under the background of improved variety heredity; then every two seeds are crossbred with each other according to the near-isogenic lines, so as to polymerize the target grain type QTL into a material to-be-improved, and obtain a genome of an improved variety and a singleplant same as the original variety except the target gene segment by screening the heredity background; meanwhile, the genetype of the target grain type QTL is judged according to a molecular marker tightly linked with the target grain type QTL with the assistant of molecular marker auxiliary selection. According to the scheme, paddy rice varieties of different grain sizes and different weights can be obtained.

Description

technical field [0001] The invention belongs to the technical field of rice molecular assisted breeding, and specifically relates to a method for obtaining materials with different grain sizes and different grain weights by using aggregated grain type QTL, and provides suitable materials for improving the appearance quality and grain weight of rice at present for improving the breeding of high-quality varieties . Background technique [0002] Yield and quality are the two main goals of rice breeding. Both thousand-grain weight and grain shape are intrinsic biological attributes of grains, among which thousand-grain weight is an important factor for yield, and grain shape is an important appearance quality trait, both of which have important economic traits. Grain type includes grain length, grain width and grain thickness traits, and grain weight is often measured by thousand-grain weight. Rice grain size is an important agronomic trait. Grain size is also an important ri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 何予卿孙亮
Owner HUAZHONG AGRI UNIV
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