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Method for enriching efficient biphenyl degradation flora by using accelerator SRpf

A technology of accelerator and degrading bacteria, which is applied in the field of microbial degradation of pollutants, can solve the problems of slow growth, low degradation efficiency of strains, and low tolerance concentration, and achieve the goal of promoting growth, increasing biphenyl degradation rate, and low cost Effect

Active Publication Date: 2013-12-11
ZHEJIANG UNIV
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Problems solved by technology

[0007] The purpose of the present invention is to provide a kind of enrichment of highly efficient biphenyl degrading bacteria group by using accelerator SRpf to solve the problems of low bacterial strain degradation efficiency, slow growth rate and low tolerance concentration in the microbial remediation technology of existing PCBs polluted environment Methods

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  • Method for enriching efficient biphenyl degradation flora by using accelerator SRpf

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Embodiment Construction

[0023] The present invention utilizes accelerator SRpf to enrich the method for high-efficiency biphenyl-degrading flora, comprising the following steps:

[0024] (1) Inoculate Micrococcus Luteus IAM14879 in LMM liquid medium to make the culture medium OD 600nm 0.05-0.2; LMM liquid medium composition is: 2.5-4.5g / L NH 4 Cl, 1.0-2.0g / L of KH 2 PO 4 , 0.005g / L biotin, 0.02g / L L-methionine, 0.04g / L vitamin B1, 0.5-1.5g / L inosine, 0.03g / L MgSO 4 , 8.0-10.0g / L L-lithium lactate, 1.5-3.0ml / L mineral salt solution (0.375g / L CuSO 4 ·5H 2 O, 0.785g / L MnCl 2 4H 2 O, 0.18g / LFeSO 4 ·7H 2 O, 0.029g / L Na 2 MoO 4 2H 2 O, 0.089g / L ZnSO 4 ·7H 2 O, the solvent is water), the pH is 6.5-8.0, cultivated for 24-36h to obtain the seed culture solution;

[0025] (2) Inoculate the seed culture solution obtained in step 1 into LMM liquid medium at an inoculation amount of 3-5% (v / v), culture at 160r / min for 48-60h, and centrifuge the fermentation broth (8000-10000r / min) 10-15min to remo...

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Abstract

The present invention discloses a method for enriching efficient biphenyl degradation flora by using an accelerator SRpf, wherein the accelerator SRpf is added to an enrichment culture process of biphenyl degradation bacterial, continuous acclimation is performed to obtain an enrichment liquid with an efficient biphenyl degradation performance, and bacterial growth, flora diversity and a biphenyl degradation rate can be significantly increased with addition of the accelerator SRpf, wherein specifically bacterial growth is increased by 30-50%, a biphenyl degradation rate is increased by 20-70%, and a Shannon's diversity index is increased to 2.10-2.34 from 1.56-1.70. The accelerator SRpf has functions of VBNC flora recovery and bacterial growth promotion, such that the accelerator SRpf can be provided for enriching efficient biphenyl degradation flora and promoting growth of other organic pollutant degradation flora so as to provide a safe, cheap and efficient method for exploration of potential degradation flora in PCB, PAH and other pollution environments.

Description

technical field [0001] The invention belongs to the field of microbial degradation of pollutants, and in particular relates to a method for enriching high-efficiency biphenyl-degrading bacterial groups by using an accelerator SRpf. Background technique [0002] Biphenyl naturally exists in coal tar, natural gas, and crude oil, and enters the environment through the combustion of fossil fuels. In industrial production, biphenyl is catalyzed by elemental iodine and ferric chloride to produce 209 homologues. This type of compound is called polychlorinated biphenyls (PCBs for short). PCBs have been widely used in transformers, capacitor insulating oil, fuel dispersants, lubricants, plasticizers and coatings due to their good flame retardancy, high insulation and thermal stability. PCBs, as one of the 12 persistent organic pollutants (POPs), have become a typical xenobiotic organic pollutant that has been widely concerned due to environmental persistence and "three pollution" ef...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/265
Inventor 苏晓梅沈超峰胡金星秦智慧黄荣浪
Owner ZHEJIANG UNIV
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