Method for enriching efficient biphenyl degradation flora by using accelerator SRpf
A technology of accelerator and degrading bacteria, which is applied in the field of microbial degradation of pollutants, can solve the problems of slow growth, low degradation efficiency of strains, and low tolerance concentration, and achieve the goal of promoting growth, increasing biphenyl degradation rate, and low cost Effect
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[0023] The present invention utilizes accelerator SRpf to enrich the method for high-efficiency biphenyl-degrading flora, comprising the following steps:
[0024] (1) Inoculate Micrococcus Luteus IAM14879 in LMM liquid medium to make the culture medium OD 600nm 0.05-0.2; LMM liquid medium composition is: 2.5-4.5g / L NH 4 Cl, 1.0-2.0g / L of KH 2 PO 4 , 0.005g / L biotin, 0.02g / L L-methionine, 0.04g / L vitamin B1, 0.5-1.5g / L inosine, 0.03g / L MgSO 4 , 8.0-10.0g / L L-lithium lactate, 1.5-3.0ml / L mineral salt solution (0.375g / L CuSO 4 ·5H 2 O, 0.785g / L MnCl 2 4H 2 O, 0.18g / LFeSO 4 ·7H 2 O, 0.029g / L Na 2 MoO 4 2H 2 O, 0.089g / L ZnSO 4 ·7H 2 O, the solvent is water), the pH is 6.5-8.0, cultivated for 24-36h to obtain the seed culture solution;
[0025] (2) Inoculate the seed culture solution obtained in step 1 into LMM liquid medium at an inoculation amount of 3-5% (v / v), culture at 160r / min for 48-60h, and centrifuge the fermentation broth (8000-10000r / min) 10-15min to remo...
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