Method for creating cabbage type rape mutant

Abstract

The invention relates to a method for creating a cabbage type rape mutant. The method comprises the following steps of sexual hybridization, young embryo rescue, rapid propagation and subculture, chromosome doubling and mutation screening. Compared with physical radiation mutagenesis, chemical mutagenesis and aerospace mutagenesis breeding methods, the method provided by the invention is environment-friendly, and cannot injury experiment operators. The technology is low in requirements for market access, can be completed in ordinary laboratories, and is low in cost.

Description

technical field [0001] The invention relates to the technical field of biological breeding, in particular to a method for creating mutants of Brassica napus. Background technique [0002] Brassica napus is one of the most important oil crops in the world, and it is also a typical material for the genetic research of cruciferous plants. Mutagenesis can generate abundant mutants and has been widely used in plant genetics and biological research. Mutagenesis technology was also applied in rapeseed breeding earlier. Through radiation, chemical mutagenesis, space loading and other methods, space loading has obtained growth period, silique length, high yield, high oil, flower shape, herbicide resistance, Different types of mutant germplasm resources such as disease resistance and leaf yellowing have enriched the gene pool and are used in variety breeding. But so far, no report has been found on the direct use of interspecific hybridization between Brassica napus and Brassica nap...

AI Technical Summary

Problems solved by technology

[0004] Although radiation mutagenesis and chemical mutagenesis can create mutants, the physical rays and chemical mutagens used will cause serious damage to the environment and the human body, and the requirements for experimental conditions and operating techniques are very strict during use. It is difficult to have physical and chemical mutagenesis conditions, and ordinary experimenters do not have physical and chemical mutagenesis techniques. Therefore, it is difficult to popularize this technique

Similarly, there are problems such as high cost and high threshold for space carrying, which makes it difficult for general laboratories to apply

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