Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting ginsenoside from American ginseng

A technology of ginsenoside and American ginseng, which is applied in the field of medicine, can solve problems such as cumbersome process, long extraction time, and loss of active ingredients, and achieve the effects of improving extraction efficiency, shortening extraction time, and promoting release

Inactive Publication Date: 2013-12-25
WEIHAI JINYIYANG PHARMA
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional extraction method has defects such as low extraction yield, loss, decomposition, and inactivation of active ingredients, high impurity content in the product, long extraction time, cumbersome process, and difficulty in large-scale production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Superfine American ginseng, pass through a 100-400 mesh drug sieve, take 1kg of American ginseng powder, add 15L of 80U / mL peroxidase, enzymatically hydrolyze at 65°C for 1h, filter, add 25L of 80% ethanol to the residue, and dissolve it at 90 Extract at ℃ for 0.5 hours, extract twice under this condition, filter and concentrate the filtrate under reduced pressure, and dry to obtain the crude ginsenoside. The content of ginsenoside Rb1 was measured by HPLC, and the extraction rate of ginsenoside Rb1 was 1.43%.

Embodiment 2

[0015] Superfine American ginseng, pass through a 100-400 mesh drug sieve, take 1kg of American ginseng powder, add 25L of 50U / mL peroxidase, enzymatically hydrolyze at 60°C for 1h, filter, add 30L of 75% ethanol to the residue, and dissolve at 90 Extract at ℃ for 0.5 hours, extract twice under this condition, filter and concentrate the filtrate under reduced pressure, and dry to obtain the crude ginsenoside. The content of ginsenoside Rb1 was measured by HPLC, and the extraction rate of ginsenoside Rb1 was 1.40%.

Embodiment 3

[0017] Grind American ginseng ultrafinely, pass through a 100-400-mesh drug sieve, take 1kg of American ginseng powder, add 20L of 70U / mL fungal laccase, enzymatically hydrolyze at 55°C for 1.5h, filter, add 30L of 75% ethanol to the residue, and dissolve it at 85 Extract at ℃ for 1 hour, extract 3 times under this condition, filter and concentrate the obtained filtrate under reduced pressure, and obtain crude ginsenoside after drying. The content of ginsenoside Rb1 was measured by HPLC, and the extraction rate of ginsenoside Rb1 was 1.41%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicine and discloses a method for extracting ginsenoside from American ginseng. The method comprises the following steps of performing superfine grinding on American ginseng, sieving with a 100-400-mesh medicinal sieve, adding a lignin degrading enzyme with the activity of 50-80U / mL for enzymolysis for 0.5-1.5h at 55-65 DEG C, filtering, adding residues into 65-80% alcohol for leaching for 2-3 times, filtering, concentrating filtrate at reduced pressure, drying, and obtaining a ginsenoside crude product. According to the method, the superfine grinding and enzyme treatment are combined, so that the efficiency and amount of extracting the ginsenoside from the American ginseng are improved and increased.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a method for extracting traditional Chinese medicines, in particular to a method for extracting ginsenosides from American ginseng. Background technique [0002] American ginseng (Panax.quinquefolium L., English name: American Ginseng), also known as American ginseng, Cantonese ginseng, American ginseng, American ginseng, is the dried root of American ginseng, a plant of Araliaceae. Ginsenoside is one of its main active components, which has anti-fatigue, hypoxia resistance, immune regulation, anti-tumor effects, among which ginsenosides Rg1, Re, and Rb1 are regarded as the representative components of American ginseng. [0003] At present, the extraction of ginsenosides mainly adopts water extraction and alcohol precipitation method, heating reflux method, microwave extraction method and ultrasonic extraction method. However, the traditional extraction method has defects such as ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/258
Inventor 孟洪涛于红霞于海先
Owner WEIHAI JINYIYANG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com