Method for preparing GAPDH (reduced glyceradehyde-phosphate dehydrogenase) transgenic cotton

A cotton, coding gene technology, applied in the fields of botany equipment and methods, biochemical equipment and methods, genetic engineering, etc., can solve the problems of undiscovered genes and complex mechanisms, and achieve the effect of improving the ability of salt and alkali tolerance
CN103468655AActive Publication Date: 2013-12-25INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Publication Date
2013-12-25

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Abstract

The invention discloses a method for preparing GAPDH (reduced glyceradehyde-phosphate dehydrogenase) transgenic cotton. GAPDH proteins are as shown in a formula (1) or (2), wherein the formula (1) represents proteins as shown in SEQ ID No.2; (2) the formula (2) represents proteins which are obtained by substitution and / or deletion and / or addition of one or a few of amino acid residues of an amino acid sequence as shown in the SEQ ID No.2. According to the invention, the GAPDH transgenic cotton is obtained for the first time, a foundation is established for cultivation of saline-alkaline tolerance cotton variety and improvement of saline-alkaline tolerance capacity of the cotton, and economic and social benefits are achieved.
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Description

technical field

[0001] The invention relates to a method for preparing transgenic GAPDH cotton. Background technique

[0002] Soil salinization is a global ecological problem and one of the main factors of land desertification and cultivated land degradation in the world today. Land salinization has a huge impact on agricultural production and ecological environment. How to develop and utilize saline-alkali land has become the focus of increasing attention. The high concentration of salt in the soil will cause ion imbalance, oxidative damage, water shortage and nutrient deficiency in the plant, and lead to the destruction of biological macromolecules, growth retardation, and even plant death, which seriously affects the growth and development of plants, resulting in crop yield reduction or failure , restricting agricultural production and development. The impact of specific salt stress on plants is manifested in: the impact on seed germination, the impact on plant photosyn...

Claims

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