A new rice grain length gene qgs3b produced by space mutagenesis and its molecular marker method

A molecular marker, rice technology, applied in the fields of biochemical equipment and methods, genetic engineering, plant genetic improvement, etc., can solve problems such as the limitations of molecular molecular breeding and utilization, achieve good appearance quality and yield components, and save the adult stage. The process of grain identification, the effect of speeding up the breeding process

Inactive Publication Date: 2016-09-07
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, the favorable alleles at these sites are all derived from natural mutations, so their application in molecular breeding is limited.

Method used

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  • A new rice grain length gene qgs3b produced by space mutagenesis and its molecular marker method
  • A new rice grain length gene qgs3b produced by space mutagenesis and its molecular marker method
  • A new rice grain length gene qgs3b produced by space mutagenesis and its molecular marker method

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Experimental program
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Effect test

Embodiment Construction

[0020] The present invention will be further described below in conjunction with specific implementation examples. The methods used herein are conventional methods unless otherwise specified.

[0021] (1) Location of qGS3b gene

[0022] 1. Test materials

[0023] A hybrid combination was constructed by using the variety "Big Grain Mutant" derived from my country's aerospace mutation and the original parent Nongken 58 to obtain F 1 F 2 Progeny of 360 individual plants and their derived F 3 family lineage.

[0024] 2. DNA extraction, PCR amplification and gel electrophoresis

[0025] Referring to the DNA extraction method of Temnykh et al. (2000), the representative individual plants of each strain were mixed and extracted with genomic DNA. According to the reference map, 600 SSR markers evenly distributed in the whole genome were selected to synthesize primers. The genomic DNA of each strain was used as a template for polymerase chain reaction (PCR). The products of the...

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Abstract

The invention relates to a new gene qGS3b for simultaneously increasing rice grain length and grain weight and a molecular marker method thereof, belonging to the fields of high-yield and high-quality rice breeding and molecular genetics. The essence of the present invention is to use the "large-grain mutant" and the F 2 groups and their derived F 3 In the family, through single-marker analysis, the new grain length gene qGS3b on the third chromosome was located, and the practical and economical marker XM3‑1 based on PCR amplification closely linked to it was obtained. Applying the present invention to auxiliary selective breeding and aggregate breeding for rice appearance quality and yield can effectively make up for the deficiency of natural variation genes in the past, and genotype selection can be performed on low-generation breeding populations at the seedling stage to obtain appearance quality and yield groups. Sort better breeding materials, save the process of grain identification at the adult stage, improve breeding efficiency, and speed up the breeding process.

Description

technical field [0001] The present invention relates to a new gene qGS3b for simultaneously increasing rice grain length and grain weight and its molecular marker method, which belongs to the fields of rice high-yield and high-quality breeding and molecular genetics, and is suitable for introducing new grain-length gene qGS3b in rice high-yield and high-quality breeding, and Assisted selection breeding and aggregation breeding for appearance quality and yield using molecular markers. Background technique [0002] Grain shape, especially grain length (GL), is an important component of rice appearance quality, while grain weight (TGW) is one of the important yield components. It is known that grain length and grain weight are affected by more than 100 genes / QTL loci, of which a small number have been finely mapped and / or cloned, mainly distributed on chromosome 2 (gw2), chromosome 3 (GS3 and GL3. 1), chromosome 5 (qSW5 / GW5), chromosome 7 (GS7 ​​and qSS7) and chromosome 8 (GW8...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 徐建龙黎志康郑天清周永力赵秀琴王文生张帆孙勇王小倩
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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