Novel lysozyme preparation as well as preparation method thereof

A technology of lysozyme and lysozyme dimer, applied in animal feed, additional food elements, animal feed, etc., can solve the problems of low absorption efficiency, low bioavailability, poor stability, etc., and improve production performance and immune function , enhance the effect of intestinal absorption

Active Publication Date: 2014-01-22
SHANGHAI E K M BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Protein is a biological macromolecule with poor stability. It must withstand the harsh physical and chemical environment of the entire digestive system when taken orally. The absorption efficiency in the body is low and the bioavailability is extremely low.
For protein macromolecules to be absorbed into the blood smoothly, two natural barriers must be overcome: one is the decomposition and degradation of acids, alkalis, and many digestive enzymes in the gastrointestinal tract; the other is the intestinal absorption barrier, and polar protein macromolecules usually cannot fully absorbed into the blood

Method used

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  • Novel lysozyme preparation as well as preparation method thereof
  • Novel lysozyme preparation as well as preparation method thereof
  • Novel lysozyme preparation as well as preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the synthesis of lysozyme dimer:

[0039] The experimental materials are egg white lysozyme and cross-linking agent DMS (Dimethyl suberimidate dihydrochloride). The synthesis is basically carried out according to the instructions of Alorich Chemistry Company. The 20K ultrafiltration membrane device was concentrated and dialyzed, and the dimer purity was 42% on average.

Embodiment 2

[0040] Example 2. Preparation of lysozyme dimer sodium alginate microspheres (Lys.D-SA-MS):

[0041]According to the routine preparation of high-pressure spray method, the main parameters of preparation are: sodium alginate concentration 2.0% (W / V), lysozyme dimer concentration 0.1% (W / V), calcium chloride concentration 3%. The prepared drug-loaded microspheres have good dispersibility and stability, the particle size range is 150-300 μm (moisture content is about 10% after drying), and the drug-loading capacity is 2.5%.

Embodiment 3

[0042] Embodiment 3, grain bud dry powder group (experimental group 1)

[0043] Make 1000g of regular feed, add 7.6g of Lys.D-SA-MS, 50g of grain bud dry powder, the mixing ratio of the two is 1:6.5, and the mice eat freely for 23 consecutive days.

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Abstract

The invention relates to preparation of a novel lysozyme oral preparation, wherein the preparation is prepared by the following steps: synthesizing lysozyme dimer proteins; preparing lysozyme dimer sodium alginate microspheres by utilizing the lysozyme dimer proteins; mixing with rice sprouts or a decoction or an adsorbent thereof. The preparation remarkably promotes the intestinal absorption efficiency of lysozyme proteins. As a novel feed additive, the preparation can replace antibiotics to enhance the production performance and nonspecific immune function of mammalian bred animals such as hogs.

Description

technical field [0001] The present invention relates to the preparation of a novel oral preparation of lysozyme, which is characterized in that it is composed of lysozyme dimer sodium alginate microspheres, grain sprouts or its decoction or its adsorbent, both of which are indispensable; the preparation is obviously Promote the intestinal absorption efficiency of lysozyme protein, as a new feed additive, it can replace antibiotics to enhance the production performance and non-specific immune function of pigs and other mammalian breeding animals. Background technique [0002] Lysozyme (Lysozyme) is a protein-based biological enzyme with antibacterial, antiviral, immune regulation, hemostasis, swelling, and accelerated tissue repair functions. It is an important non-specific immune factor and has a wide range of applications in the field of medicine Applications. Lysozyme dimer is the dimer form of lysozyme after synthesis. In recent years, it has been found that in addition...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K1/17A23K20/195
Inventor 陈宇光黄岩姚雪丽朱振华苏洁戴小峰
Owner SHANGHAI E K M BIOTECH
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