Special gas accurate controller for cell exposure

Abstract

The invention provides a special gas accurate controller for cell exposure. The special gas accurate controller comprises a stirring mixer, wherein the stirring mixer is provided with three accurate gas flow controlling valves which are connected with a compressed air cylinder, a carbon dioxide gas cylinder and a special equalization gas cylinder, the outer surface of each of the compressed air cylinder, the carbon dioxide gas cylinder and the special equalization gas cylinder is provided with a temperature controlling sleeve, two ends of the special equalization gas cylinder are provided with a vacuum stop valve and an equalization stop valve, the rear end, provided with an electrically controlled three-way valve, of the equalization stop valve is connected with a vacuum pump, the other end of the equalization stop valve is connected to a special total gas source by virtue of a total gas source stop valve, one end of the special equalization gas cylinder is provided with a swing shaft, the swing shaft is connected to an eccentric gear by virtue of a rocker, an output pipeline of the stirring mixer is connected to an exposure gas hole arranged in an exposure gas bin by virtue of an exposure gas flow controlling valve and carries out a quantitative exposure test on the cell on a cell film plate, and a computer controller is used for controlling all electronic valves, motors and total temperature controllers according to a preparatory program to reach the purpose of accurately controlling the special gas during cell exposure. The special gas accurate controller has the beneficial effect of accurately controlling the special gas to carry out scientific research and scientific experiments of cell exposure.

Description

technical field [0001] The invention provides a precise control device for cell exposure to a specific gas. Background technique [0002] At present, in the fields of medicine, environmental science, military science, toxicology and other scientific fields, the study of the impact of some artificially synthesized specific gases on humans has penetrated into the field of molecular science. In order to conduct research in this area, specially cultivated cells or microorganisms are often used Inoculate in advance on a special cell membrane plate as the subject of the test, and apply different doses and concentrations of specific gases on the cell membrane plate to conduct exposure experiments on cells or microorganisms, because experiments on such cells or microorganisms require precise application of specific gases The flow, dosage and concentration of the gas flow rate and the concentration of carbon dioxide and air are required to be contained in a certain proportion. Since ...

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Problems solved by technology

[0002] At present, in the fields of medicine, environmental science, military science, toxicology and other scientific fields, the study of the impact of some artificially synthesized specific gases on humans has penetrated into the field of molecular science. In order to conduct research in this area, specially cultivated cells or microorganisms are often used Inoculate in advance on a special cell membrane plate as the subject of the test, and apply different doses and concentrations of specific gases on the cell membrane plate to conduct exposure experiments on cells or microorganisms, because experiments on such cells or microorganisms require precise application of specific gases The flow, dosage and concentration of the gas flow rate and the concentration of carbon dioxide and air are required to be contained in a certain proportion. Since there is no special precise control equipment and method to meet the above requirements, the simple manual operation is inaccurate, the ratio of various gases is uneven, and it is also affected by the ambient temperature. Harsh experimental conditions, such as impact, bring great difficulty to the operation. These problems make the scientific research and scientific research experiments on cells exposed to specific gases have to stay at the stage of theoretical speculation or manual rough experiments, and cannot further complete rigorous sh experiments and experiments. Theoretical demonstration severely restricts the application and development of cell exposure to specific gas toxicology in scientific research in this field, so a special cell that can precisely control the flow, dose and concentration of specific gas while ensuring safety protection and cell growth environment is developed There is an urgent need for experimental equipment that exposes precise control devices for specific gases

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