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Preparation method of chitosan oligosaccharide nanoparticle

A technology of chitosan oligosaccharide and nanoparticles, which is applied in the directions of non-active ingredients medical preparations, pharmaceutical formulations, non-central analgesics, etc., can solve problems such as limiting wider application, and achieve increased drug loading and long drug release. Good effect of time and affinity

Inactive Publication Date: 2014-01-29
NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its structure and molecular weight, the water solubility of chitosan limits its wider application

Method used

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  • Preparation method of chitosan oligosaccharide nanoparticle
  • Preparation method of chitosan oligosaccharide nanoparticle
  • Preparation method of chitosan oligosaccharide nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 0.1g chitosan oligosaccharide was dissolved in 6ml double distilled water, 0.06g ibuprofen and 0.4g EDC were dissolved in acetone. Add the acetone solution of ibuprofen and EDC to the double distilled aqueous solution of chitosan oligosaccharide under rapid stirring, and react at a constant temperature of 25° C. in a water bath for 36 hours under magnetic stirring. The resulting product was dialyzed with double distilled water for 12 hours in a semipermeable membrane, then dialyzed with absolute ethanol for 12 hours, then dialyzed with double distilled water for 24 hours, finally centrifuged and freeze-dried to obtain chitosan-ibuprofen conjugates . Mix equal concentrations of paclitaxel in acetone and oligochitosan-ibuprofen conjugate aqueous solution at a ratio of 1:10, ultrasonically shake for 40 minutes, centrifuge, and freeze-dry to obtain paclitaxel-loaded oligochitosan-ibuprofen nanoparticles .

Embodiment 2

[0037] 0.1g chitosan oligosaccharide was dissolved in 6ml double distilled water, 0.04g ibuprofen and 0.3g EDC were dissolved in absolute ethanol. Add the absolute ethanol solution of ibuprofen and EDC to the double distilled aqueous solution of chitosan oligosaccharide under rapid stirring, and react at a constant temperature of 25° C. in a water bath for 53 hours under magnetic stirring. The resulting product was dialyzed with double distilled water for 24 hours in a semipermeable membrane, then dialyzed with absolute ethanol for 24 hours, then dialyzed with double distilled water for 24 hours, finally centrifuged and freeze-dried to obtain chitosan-ibuprofen conjugates . Mix an equal concentration of curcumin ethanol solution and chitosan oligosaccharide-ibuprofen conjugate aqueous solution at a ratio of 1:2, ultrasonically vibrate for 30 minutes, centrifuge, and freeze-dry to obtain curcumin-loaded chitosan-ibuprofen nanoparticles.

Embodiment 3

[0039]0.1g chitosan oligosaccharide was dissolved in 10ml double distilled water, 0.06g ibuprofen and 0.2g EDC were dissolved in absolute ethanol. Add the absolute ethanol solution of ibuprofen and EDC to the double-distilled aqueous solution of chitosan oligosaccharide under rapid stirring, and react at a constant temperature of 30° C. in a water bath for 30 hours under magnetic stirring. The resulting product was dialyzed with double distilled water for 24 hours in a semipermeable membrane, then dialyzed with absolute ethanol for 24 hours, then dialyzed with double distilled water for 24 hours, finally centrifuged and freeze-dried to obtain chitosan-ibuprofen conjugates . Mix an equal concentration of curcumin ethanol solution and chitosan oligosaccharide-ibuprofen conjugate aqueous solution at a ratio of 1:6, ultrasonically vibrate for 20 minutes, centrifuge, and freeze-dry to obtain curcumin-loaded chitosan-ibuprofen nanoparticles.

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Abstract

The invention discloses a preparation method of a chitosan oligosaccharide nanoparticle. When a conjugate formed by coupling chitosan oligosaccharide which is good in biocompatibility and biodegradable with an analgesic medicament ibuprofen is dispersed in water, hydrophobic ibuprofen is self-assembled to form a core of the nanoparticle, and hydrophilic chitosan oligosaccharide is self-assembled to form a spherical shell, so that the chitosan oligosaccharide-ibuprofen nanoparticle is formed. The ibuprofen has good hydrophobicity, and therefore, the obtained nanoparticle has a smaller average particle size and relatively uniform distribution. The hydrophobic core of the chitosan oligosaccharide-ibuprofen nanoparticle has a good affinity action on the hydrophobic medicament, and the loaded hydrophobic anti-tumor medicament can be used for improving targeting, hypotoxicity and slow release effect of the medicament. The chitosan oligosaccharide-ibuprofen nanoparticle loaded with the hydrophobic anti-tumor medicament has a good slow release effect. And meanwhile, the ibuprofen in the nanoparticle can be used for achieving an analgesic effect.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to a preparation method of chitosan oligosaccharide nanoparticles. Background technique [0002] The nanoparticle drug-loading system has high drug-loading capacity, controllable particle size, and permeability. It can release drugs slowly and prolong the drug action time; solubilize hydrophobic drugs to promote their absorption and improve bioavailability; Treatment of special parts; on the premise of ensuring the effect of the drug, change the distribution characteristics of the drug in vivo, reduce the dosage, thereby reducing or avoiding adverse reactions; improve the stability of the drug, which is conducive to storage; protect biological substances such as nucleotides and proteins Macromolecular drugs can prevent the inactivation of DNA and protein drugs, and can help nucleotide transfection cells, greatly improving the transfection rate; changing the membrane trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/48A61K9/19A61K9/08A61K31/192A61P29/00A61P35/00A61K31/12
Inventor 刘占军吴振刚赵鸣飞左桂福李慧兰陈艳华韩刚劳凤云
Owner NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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