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Space bacillus cereus strain LCT-BC25

A LCT-BC25, Bacillus cereus technology, applied in bacteria, microorganisms, biochemical equipment and methods, etc., can solve problems such as limitations, Bacillus cereus research has not yet been reported, etc.

Inactive Publication Date: 2014-09-17
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Previous studies on the biological characteristics and uses of Bacillus cereus were limited to the ground, and research on Bacillus cereus in the space environment has not been reported

Method used

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  • Space bacillus cereus strain LCT-BC25
  • Space bacillus cereus strain LCT-BC25
  • Space bacillus cereus strain LCT-BC25

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0016] The experimental methods used in the following implementation examples are conventional methods unless otherwise specified.

[0017] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0018] 1. Phenotype of Bacillus cereus LCT-BC25

[0019] 1. Strain selection: Bacillus cereus LCT-BC25 comes from China General Microorganism Culture Collection Center, and the preservation number is CGMCC NO.6515.

[0020] 2. Morphology: The original sample was diluted and spread, and then Gram stained. Bacillus cereus LCT-BC25 is a Gram-positive bacillus with spores arranged singly or in pairs.

[0021] 3. Identification of 16s rDNA of Bacillus cereus LCT-BC25 strain

[0022] 16S rDNA is the gene of 16S rRNA sequence, about 1.5kb in length. The 16S rDNA was directly amplified by bacterial liquid PCR from the purified single bacteria, and some single bacteria that were difficult to amplify by bacterial liquid...

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Abstract

The invention relates to a spatial strain bacillus cereus LCT-BC25, more specifically to a space microorganism obtained by using a space technology. The spatial strain bacillus cereus LCT-BC25 is characterized in that the bacillus cereus strain LCT-BC25 is gram-positive bacilli, has spores, and presents enhanced drug resistance for amikacin. According to the present invention, in the levels of genome, transcriptome and proteome, the spatial strain bacillus cereus LCT-BC25 is clarified in detail, it is confirmed that the total length of the whole genome sequence of LCT-BC25 is 5,154,512 bp and the spatial strain bacillus cereus LCT-BC25 has 5,263 coding genes, 310 up-regulation genes and 996 down-regulation genes, it is confirmed that biological functions of the genes are mainly related to physiological processes of cell growth and repair, the spatial strain bacillus cereus LCT-BC25 has 57 expression up-regulated proteins and 77 expression down-regulated proteins, and it is confirmed that functions of differential expression protein are mainly related to carbohydrate metabolism, lipid metabolism and amino acid metabolism.

Description

technical field [0001] The invention belongs to the field of microbes and biotechnology, and relates to a new microbial strain Bacillus cereus LCT-BC25 and its biological characteristics and genome DNA sequence. Transcriptome and differential proteomics analysis was carried out by using bioinformatics technology, and obtained Its characterization in genome, transcriptome and proteome. Background technique [0002] With the increasing frequency of human space exploration activities, space has become an important field of human activities. Microorganisms are part of the natural environment, and they exist in various parts of the biosphere such as air, water, and soil. Human space activities inevitably bring microorganisms into space. Despite the extreme and complex environment of outer space, these microorganisms are able to adapt to these changes such as microgravity, cosmic rays, temperature, pressure, etc. and exhibit corresponding changes in morphology and physiology. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/085
Inventor 刘长庭苏龙翔方向群王俊峰李天志郭英华常德王雅娟陈振鸿王立
Owner GENERAL HOSPITAL OF PLA