Bacillus cereus with heavy metal passivating and plant growth promoting functions and application of bacillus cereus

A Bacillus cereus, plant growth-promoting technology, applied in plant growth regulators, plant growth regulators, applications, etc., to achieve the effect of reducing soil available cadmium

Active Publication Date: 2016-09-07
广州秦安农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus cereus which has the functions of heavy metal pa

Method used

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  • Bacillus cereus with heavy metal passivating and plant growth promoting functions and application of bacillus cereus
  • Bacillus cereus with heavy metal passivating and plant growth promoting functions and application of bacillus cereus
  • Bacillus cereus with heavy metal passivating and plant growth promoting functions and application of bacillus cereus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Isolation and purification of embodiment 1 bacterial strain

[0036] (1) Separation

[0037] ①Preliminary screening

[0038] Weigh 10.0 g of sugarcane field soil in Shibao, Maba Town, Qujiang District, Shaoguan City, Guangdong Province, put it into a 250 mL Erlenmeyer flask filled with 90 mL of sterile water (with glass beads), shake at 25°C and 200 r / min for 30 min, Stand still for 20-30s, take 10mL of the supernatant and place it in a 50mL sterilized Erlenmeyer flask, put it in a water bath at 90°C for 10min, take 1mL of the supernatant after the water bath and add it to the insoluble inorganic phosphorus medium, (150mL of the Erlenmeyer flask contains 30mL of culture liquid), and two replicates were set for each soil sample. Place on a shaker for enrichment culture, with a rotation speed of 150r / min and a temperature of 30°C. The enrichment culture period is 5-7 days. After the first enrichment culture, take 1 mL of the culture solution and add them to the new med...

Embodiment 2

[0043] Example 2 Characterization

[0044] The B19 bacteria screened in Example 1 were subjected to physiological and biochemical identification and molecular biological identification.

[0045] (1) Morphological characteristics of bacteria

[0046] B19 bacteria are Gram-positive bacteria with rod-shaped cells and square ends, short or long chains, large colonies, rough, flat, and irregular surfaces. Gram-stained micrographs of bacteria figure 1 As shown, the cells appear purple. Spore malachite green dye staining, micrograph as figure 2 As shown, the spores appear green.

[0047] B19 colonies formed after cultured on beef extract peptone medium for 24 hours were round or nearly round, soft, colorless, and slightly glossy white colonies.

[0048] (2) Growth characteristics

[0049] In the liquid culture medium, under the conditions of rotating speed 180rpm, temperature 37℃, and initial pH value 7.0, cultured for 18 hours, and the number of viable bacteria measured was 3...

Embodiment 3

[0058] Example 3 Effect experiment of B19 bacteria producing water-soluble phosphorus under insoluble inorganic phosphorus medium culture

[0059] (1) Preparation of test solution

[0060] Pick B19 cells stored on beef extract peptone slant medium, streak on LB solid medium plate, and culture at 30°C for 24h. Transfer the B19 bacteria on the plate into a 150 mL Erlenmeyer flask containing 20 mL of LB liquid medium, and culture on a shaker at 30°C, 150 r / min, for 24 hours. Centrifuge the culture solution at 6000r / min for 5min, resuspend the bacteria with sterile water, spread evenly on the vortex mixer, transfer the same amount of the bacteria suspension into a 150mL Erlenmeyer flask containing 30mL inorganic phosphorus screening medium with a pipette gun , 30°C, 150r / min, shaker for 7 days.

[0061] (2) Determination of water-soluble phosphorus content

[0062] The culture solution was centrifuged at 10000g, 25°C for 15min, and the supernatant was taken, and the content of ...

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Abstract

The invention discloses bacillus cereus with heavy metal passivating and plant growth promoting functions and application of the bacillus cereus, and belongs to the technical field of microbial strains. The name of the bacillus cereus is bacillus cereus B19, and is preserved in the China General Microbiological Culture Collection Management Committee Centre of Institute of Microbiology, Chinese Academy of Sciences, 3#, No.1 Yard, West Beicheng Road, Chaoyang District, Beijing, on April 27th, 2016; the preservation number is CGMCC NO. 12405. The bacillus cereus has the functions of passivating heavy metals and promoting plant growth, is suitable for decomposing difficultly soluble phosphorus, and is used for producing auxin, resisting salt, resisting the heavy metals, promoting plant seed germination, increasing effective phosphorus and microbial carbon and nitrogen of soil, and reducing available heavy metals in the soil.

Description

technical field [0001] The invention belongs to the technical field of microbial strains, and in particular relates to a bacillus cereus with the functions of passivating heavy metals and promoting plant growth and its application. Background technique [0002] Phosphorus is one of the essential mineral elements for plant growth. It participates in various physiological and biochemical processes in plants in various ways, such as energy metabolism, photosynthesis, respiration and nitrogen fixation. Generally, the total phosphorus content in farmland soil is very high, but the available phosphorus content is very low. More than 95% of the phosphorus in the soil in our country is invalid phosphorus, which cannot be absorbed and utilized by plants. Therefore, phosphorus can only be absorbed and utilized by plants through external sources; and the water-soluble phosphorus fertilizer applied artificially is easily absorbed by Ca and Fe in the soil. , Al combine to form insoluble...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C09K17/14B09C1/10C12R1/085
CPCA01N63/00B09C1/10C09K17/14C12N1/205C12R2001/085
Inventor 李永涛徐会娟蒲强彭桂香蔡燕飞张玉龙
Owner 广州秦安农业科技有限公司
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