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Growth-promoting microbial inoculum capable of increasing soybean oil content and preparation method and application thereof

A growth-promoting bacterial agent, soybean technology, applied in biochemical equipment and methods, microorganism-based methods, botanical equipment and methods, etc., can solve the problems of not being able to simultaneously improve the quality index of soybean oil content, and unable to ensure the beneficial functions of strains. , to improve soybean yield and quality, save energy consumption, increase phosphorus and potassium content

Active Publication Date: 2021-10-22
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary microbial fertilizer bacterial agents can only provide the nutrients needed for soybean growth, but cannot simultaneously increase the important quality index of soybean oil content, and cannot guarantee that the strain can survive for a long time in the plant rhizosphere and perform stable beneficial functions

Method used

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  • Growth-promoting microbial inoculum capable of increasing soybean oil content and preparation method and application thereof
  • Growth-promoting microbial inoculum capable of increasing soybean oil content and preparation method and application thereof
  • Growth-promoting microbial inoculum capable of increasing soybean oil content and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Screening of strains

[0026] Select healthy soybean plants, dig out the soybean roots and soil as a whole, remove larger soil clods and other useless residues, take 1g of rhizosphere soil from each sample in sterile saline containing nystatin, and place in a shaker. Bed 160r·min -1 Shake for 0.5h to obtain the rhizosphere soil bacteria suspension. Take 1 mL of the above bacterial suspension into 9 mL of sterile saline containing nystatin, and dilute to 10 -7 concentration. Spread the above-mentioned bacterial suspension on the phosphorus-solubilizing and potassium-solubilizing solid medium containing nystatin, repeat each concentration three times, and incubate at a constant temperature of 28°C for more than 3 days until a transparent circle appears.

[0027] The formula of above-mentioned phosphate-dissolving medium is: glucose 10g, K 2 HPO 4 2.0g, ammonium sulfate 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, C...

Embodiment 2

[0034] Determination of Growth-promoting Ability of DW1 Strain

[0035]The growth-promoting bacteria with specific affinity to soybean were screened from the soybean rhizosphere by selective medium and soybean lectin double screening method, and the phosphorus-dissolving, potassium-dissolving and IAA-producing abilities of these strains were determined. The strains were made into a bacterial suspension, inoculated into different phosphorus-dissolving and potassium-dissolving mediums, and incubated at 28°C, 160r min -1 Shaking culture 5d.

[0036] The formula of the phosphate-solubilizing medium is the same as in Example 1, except that the agar is removed; the soluble phosphorus content in each culture solution is determined by the molybdenum-antimony resistance method.

[0037] Potassium solution medium formula is Na 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.5g, FeCl 3 0.005g, CaCO 3 0.1g, potassium feldspar 1.0g, glucose 5.0g, distilled water 1000mL, pH 7.0-7.5. And at 28°C, ...

Embodiment 3

[0045] Identification of strains

[0046] Combined with morphological observation, physiological and biochemical tests and 16SrRNA gene sequence comparison analysis, the species of DW1 strain was identified.

[0047] According to the "Common Bacteria System Identification Manual" and "Bergey's Bacterial Identification Manual", the physiological and biochemical characteristics of the DW1 strain were detected. Morphological tests include Gram staining; physiological and biochemical tests include starch hydrolysis test, contact enzyme test, VP test, methyl red (M-R) test, gelatin liquefaction test, glucose oxidation fermentation test, H production 2 S test, the results are shown in Table 2.

[0048] The 16S rRNA gene sequence of the DW1 strain was submitted to the NCBI database for BLAST comparison, combined with the results of physiological and biochemical tests, it was determined that the DW1 strain belonged to the genus Bacillus sp., and the 16S rRNA gene sequence of the DW1 ...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a growth-promoting microbial inoculum capable of increasing the content of soybean oil and a preparation method and application thereof. The microbial inoculum is formed by fermentation with a DW1 strain, which is classified and named as Bacillus sp., is preserved in the China Center for Type Culture Collection on July 16, 2021, and has the preservation number of CCTCC NO:M 2021889. The growth-promoting microbial inoculum disclosed by the invention has the capability of dissolving insoluble phosphorus and insoluble potassium, can increase the content of rapidly available phosphorus and rapidly available potassium in soil, has an obvious promoting effect on soybean growth, and improves the soybean yield. The growth-promoting microbial inoculum can also significantly increase the soybean oil content, has soybean agglutinin affinity, and can be subjected to an agglutination reaction with soybean agglutinin. The DW1 strain can be colonized at the roots of soybeans for a long time under mediation of lectin, and the growth promoting effect is stably achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial growth promotion, and in particular relates to a soybean growth-promoting bacteria agent capable of increasing soybean yield and oil content and dissolving phosphorus and potassium, as well as its preparation method and application. Background technique [0002] Soybean is an important grain and oil crop in my country, providing a large amount of raw materials for agriculture and industry. Phosphorus and potassium are of great significance to soybean yield and quality. Phosphorus participates in important life activities such as photosynthesis and respiration of soybeans. Phosphorus deficiency can lead to slow growth of soybeans, yellowing of leaves, and delayed flowering and maturity; potassium can activate various enzyme activities in cells and participate in maintaining the osmotic pressure balance of plant cells , enhance plant resistance to cold, salt, lodging, etc., and increase CO 2 Ass...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01C1/06A01C21/00A01C1/00A01N63/22A01N43/38C09K17/14A01P21/00C12R1/07C09K101/00
CPCC12N1/20A01C1/06A01C21/00A01C1/00A01N63/22A01N43/38C09K17/14C09K2101/00Y02A40/10A01P21/00C12N1/205C12R2001/07
Inventor 曹媛媛艾文峰黎权德郭婷婷欧康苗蔡柯邱丽娟王晓波柳佳阮鑫薛媛元霍宇蓓邓瑞宁
Owner ANHUI AGRICULTURAL UNIVERSITY
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