Articular cartilage microstructure nanoindentation inlaying and water-retaining sample stage and experiment method

A technology of articular cartilage and nano-indentation, which is used in the preparation of test samples and the application of stable tension/pressure to test the strength of materials. It can solve the problems of cartilage surface dehydration and difficult surface treatment of cartilage samples, and achieve mechanical properties. reliable results

Inactive Publication Date: 2014-02-12
BEIJING UNIV OF TECH
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nano-indentation inlaid water-retaining sample table of articular cartilage microstructure of the present invention can solve the difficulty in surface treatment of cartilage samples and the dehydration of cartilage surface by the inlay method, so that the entire tested sample does not have any slight disturbance in the surface treatment and testing process

Method used

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  • Articular cartilage microstructure nanoindentation inlaying and water-retaining sample stage and experiment method
  • Articular cartilage microstructure nanoindentation inlaying and water-retaining sample stage and experiment method
  • Articular cartilage microstructure nanoindentation inlaying and water-retaining sample stage and experiment method

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Experimental program
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Embodiment 1

[0018] Such as figure 2 Shown are the test results of the static mechanical properties of the articular cartilage microstructure, and the mechanical properties of the rabbit articular cartilage microstructure were tested using the nano-indentation mosaic water-retaining sample platform of the articular cartilage microstructure. Cut the rabbit femur including the articular cartilage into a section 2 cm high, mix bisphenol A and epichlorohydrin at a volume ratio of 1:1, stir well, pour it into the holding tank, insert the articular subchondral bone into the resin, and clamp it with tweezers The ligament on the articular cartilage adjusts the height of the articular cartilage so that it is 1mm higher. Keep it for ten minutes and wait for the resin to solidify. Turn the table body upside down on 4000-grit sandpaper, finely grind for 2 minutes, turn 6000-grit sandpaper to polish for 2 minutes; rotate the sleeve so that the sleeve is 2 mm higher than the upper edge of the table bod...

Embodiment 2

[0019] Such as image 3 Shown are the test results of the dynamic mechanical properties of the articular cartilage microstructure. Nano-indentation method was used to detect the mechanical properties of the rabbit articular cartilage microstructure. Mix 1:1, stir evenly, pour into the holding tank, insert the articular subchondral bone into the resin, clamp the articular cartilage ligament with tweezers, adjust the height of the articular cartilage to be 1mm higher; keep it for ten minutes, and wait for the resin to solidify Grind the table body upside down on 4000-mesh sandpaper for 2 minutes, turn to 6000-mesh sandpaper and polish for 2 minutes; rotate the sleeve so that the sleeve is 2 mm higher than the upper edge of the table body; install the tested sample table into the nanoindentation instrument On the card table, select the relevant method of dynamic mechanical performance test, locate the point to be tested, the maximum loading frequency is 50 Hz, and measure 20 poin...

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Abstract

The invention relates to an articular cartilage microstructure nanoindentation inlaying and water-retaining sample stage and an experiment method. The sample stage is composed of a stage body, a containing groove, sleeve, an articular cartilage and a resin. The articular cartilage microstructure nanoindentation inlaying and water-retaining sample stage can solve the problems that the surface of a cartilage sample is difficult to treat and the surface of the cartilage is dehydrolyzed when an inlaying method is adopted, so that the whole tested sample does not have any micro disturbance in the surface treatment and test processes. The sample stage treats the surface of the cartilage in a grinding and polishing manner, and can effectively show the microstructure of the surface of the cartilage; and the sample can not cause disturbance in the grinding and polishing process and the test process. All the existing nanoindenters in China basically depend on import, and water-retaining assemblies thereof are high in cost. The experiment method lowers the experiment cost, and the credibility is higher than that of an assembly configured on the nanoindenter.

Description

technical field [0001] The invention relates to an articular cartilage microstructure nano-indentation inlaid water-retaining sample platform and an experimental method, belonging to the technical field of microscale mechanical performance testing, and in particular to an articular cartilage microstructure nano-indentation inlaid water-retaining sample platform and an experimental method. Background technique [0002] At present, the mechanical performance test of articular cartilage based on nanoindentation method has become an effective method to study the performance of cartilage. However, the moisturizing method of articular cartilage in the nano-indentation experiment is poor, and it can only obtain the mechanical properties of the surface layer of cartilage. High practical value. [0003] The existing nano-indentation articular cartilage sample table can only test the mechanical properties of the cartilage surface, but cannot obtain the mechanical properties of the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N3/08G01N1/32
Inventor 杨庆生刘志远李熙杨忠军
Owner BEIJING UNIV OF TECH
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