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Cerebroprotein hydrolysate in piracetam and cerebroprotein hydrolysate tablets and preparation method of cerebroprotein hydrolysate

A cerebroprotein hydrolysate and piracetam technology, applied in the field of biological preparations, can solve the problems of polypeptide loss, low preparation efficiency, amino acid loss and the like, and achieve the effects of high yield, high economic benefit and simple operation

Active Publication Date: 2014-03-26
HONGMEI PHARMA CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For example, the Chinese patent application with application number 94104516.1 discloses a preparation method of cerebroprotein hydrolyzate, which uses steps such as homogenization, degreasing, air-drying, enzymatic hydrolysis, ultrafiltration, ion exchange, etc. The air-drying step in this method will cause substances such as polypeptides The loss of; application numbers are 01130523.1, 200510064567.6, 200710055421.4 and other Chinese patent applications disclose the new preparation method of cerebroprotein hydrolyzate, all there is the step that makes amino acid loss in a large amount in the above-mentioned method, therefore, above-mentioned method makes discussion; application number is 200610065169.0 In the process of preparing cerebroprotein hydrolyzate, exogenous amino acids are added, which is also the basic situation of cerebroprotein hydrolyzate drugs currently in clinical use, indicating that the existing method needs further improvement; Chinese patent application number 200410022091.5 discloses a A preparation method of a cerebroprotein hydrolyzate preparation, the method is to directly hydrolyze the brain tissue homogenate with protease, and obtain cerebroprotein hydrolyzate through further processing. The disadvantage of this process is that the brain tissue homogenate contains a large amount of fat, It affects the hydrolysis effect of protease, and the preparation efficiency is low. At the same time, more fatty substances will be mixed in the final brain protein hydrolyzate, which will affect the appearance and stability of the final drug

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the preparation of the cerebroprotein hydrolyzate in Piracetam cerebroprotein hydrolyzate sheet

[0044] (1) Take fresh and healthy pig brains, slow down naturally until there is no ice residue, remove impurities, and get clean pig brains;

[0045] (2) Take 10kg of clean porcine brain, add 10L of water for injection, and use a high-pressure homogenization pump to homogenate for 2 minutes each time to obtain a homogenate of brain tissue;

[0046] (3) Add 2.5 times the amount of acetone to the brain tissue homogenate, stir for 25 minutes, let stand for 3.5 hours, filter to obtain acetone residue; then add 1.5 times the amount of acetone to the acetone residue, stir for 25 minutes, let stand for 3.5 hours, filtered, and the filter residue was dried at 60°C for 3 hours to obtain cerebroprotein powder;

[0047] (4) Take trypsin and anhydrous calcium chloride (the amount of anhydrous calcium chloride is 12wt% of the amount of trypsin) and dissolve them in cold ...

Embodiment 2

[0051] Embodiment 2, the preparation of the cerebroprotein hydrolyzate in Piracetam cerebroprotein hydrolyzate sheet

[0052] (1) Take fresh and healthy pig brains, slow down naturally until there is no ice residue, remove impurities, and get clean pig brains;

[0053] (2) Take 10kg of clean porcine brain, add 10L of water for injection, and use a high-pressure homogenization pump to homogenate for 2 minutes each time to obtain a homogenate of brain tissue;

[0054] (3) Add 3.5 times the amount of acetone to the brain tissue homogenate, stir for 35 minutes, let stand for 4.5 hours, filter to obtain acetone residue; then add 2.5 times the amount of acetone to the acetone residue, stir for 35 minutes, let stand for 4.5 hours, filtered, and the filter residue was dried at 70°C for 5 hours to obtain cerebroprotein powder;

[0055] (4) Take trypsin and anhydrous calcium chloride (the amount of anhydrous calcium chloride is 13wt% of the amount of trypsin) and dissolve them in cold ...

Embodiment 3

[0059] Embodiment 3, the preparation of the cerebroprotein hydrolyzate in Piracetam cerebroprotein hydrolyzate sheet

[0060] (1) Take fresh and healthy pig brains, slow down naturally until there is no ice residue, remove impurities, and get clean pig brains;

[0061] (2) Take 10kg of clean porcine brain, add 10L of water for injection, and use a high-pressure homogenizer pump to homogenate for 2.5 minutes each time to obtain a homogenate of brain tissue;

[0062] (3) Add 3 times the amount of acetone to the brain tissue homogenate, stir for 30 minutes, let stand for 4 hours, filter to obtain acetone residue; then add 2 times the amount of acetone to the acetone residue, stir for 30 minutes, let stand for 4 hours hours, filtered, and the filter residue was dried at 65°C for 4 hours to obtain cerebroprotein powder;

[0063] (4) Take trypsin and anhydrous calcium chloride (the amount of anhydrous calcium chloride is 12.5wt% of the amount of trypsin) dissolved in cold purified ...

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Abstract

The invention belongs to the field of biological agents, and relates to cerebroprotein hydrolysate in piracetam and cerebroprotein hydrolysate tablets and a preparation method of the cerebroprotein hydrolysate, in particular to cerebroprotein hydrolysate which is extracted from brain tissues of mammals excluding human and contains polypeptide and multiple amino acids including free amino acids and free glutamic acid, as well as a preparation method of the cerebroprotein hydrolysate. The provided method comprises steps such as homogenate, two times of degreasing of acetone, enzymolysis of trypsin and pepsin and the like. The content of amino acids in the cerebroprotein hydrolysate prepared with the method is remarkably higher than that in the prior art.

Description

technical field [0001] The present invention relates to a cerebroprotein hydrolyzate in a piracetam cerebroprotein hydrolyzate sheet and a preparation method thereof, in particular to a cerebroprotein hydrolyzate extracted from the brain tissue of mammals other than humans and containing free The cerebroprotein hydrolyzate of various amino acids including amino acids and free glutamic acid, and the preparation method of the cerebroprotein hydrolyzate belong to the field of biological preparations. Background technique [0002] Brain tissue is rich in protein. For a long time, people have been studying the hydrolysis of protein in animal brain tissue into amino acids and low-molecular-weight peptides, which are used to treat dysfunction caused by insufficient cerebrovascular compensation, stroke, brain memory impairment and traumatic brain injury. functional disorders, sequelae of concussion and other diseases. [0003] Cerebroprotein hydrolyzate is a mixture rich in various...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K35/30A61P9/10A61P25/00
Inventor 郭智华
Owner HONGMEI PHARMA CHINA
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