Coronary heart disease-related genes and reagents, preparations or kits and applications thereof for in vitro detection
An in vitro detection, coronary heart disease technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve problems such as unsatisfactory coronary heart disease effect
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Embodiment 1
[0023] Example 1, a gene related to coronary heart disease is COL4A1 gene, the nucleotide sequence of the gene has the sequence shown in SEQ ID NO: 1. The test sample containing the gene of COL4A1 can be obtained from cells from the tester, such as cells from blood, urine, saliva, gastric juice, hair, biopsy and autopsy materials, preferably from blood.
Embodiment 2
[0024] Embodiment 2, a reagent for in vitro detection of coronary heart disease-related genes, the reagent is used to detect the polymorphism of COL4A1 gene rs605143 site, the reagent includes the following primers:
[0025] Upstream primer: 5'AAAGCCATTGCTACCTCA3';
[0026] Downstream primer: 5'CTGCTCCTGGTGACTCTG3';
[0027] The so-called "gene polymorphism" refers to the difference in the nucleotide sequence of each individual gene among the population. Those of ordinary skill in the art know that the polymorphic site described in the present invention is a single nucleotide polymorphic (SNP) site, that is, a single nucleotide change in the genome sequence; a difference in nucleotide sequence It can be embodied at the DNA level or at the RNA level. Therefore, the coronary heart disease-related gene of the present invention can be embodied at the DNA level, RNA level, preferably at the DNA level, more preferably at the genomic DNA level.
[0028] There are currently two stra...
Embodiment 3
[0030]Example 3, as a preference of the above example, the reagent is a reagent for combining polymerase chain reaction and restriction fragment length polymorphism analysis or a reagent for combining polymerase chain reaction and direct sequencing. The reagents for detecting the rs605143 site in the kit vary according to different detection methods. For example, when using a combination of polymerase chain reaction and restriction fragment length polymorphism analysis to detect the rs605143 polymorphic site, the kit contains Restriction enzymes and corresponding restriction enzymes may be included, such as DraI restriction enzymes and corresponding restriction enzymes.
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