Lilium oriental hybrid tissue culture seedling strengthening method

A technology of lily group and culture medium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of improving seedling quality, reducing pollution rate, and easy operation

Active Publication Date: 2014-04-02
HANGZHOU LANDSCAPING
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there is no report about the application of humic acid in lily tissue culture.

Method used

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  • Lilium oriental hybrid tissue culture seedling strengthening method
  • Lilium oriental hybrid tissue culture seedling strengthening method
  • Lilium oriental hybrid tissue culture seedling strengthening method

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Embodiment Construction

[0035] The present invention will be further explained below in combination with specific embodiments.

[0036] The humic acid in the present invention is purchased from Shanghai Jingchun Biochemical Technology Co., Ltd., the brand is Aladdin, containing fulvic acid (FA) ≥ 90%, the product number is H108498, and the content of active functional groups is high.

[0037] (1) Select the test-tube seedlings of the robust Oriental lily 'Sorbonne'.

[0038] (2) Some of the test-tube seedlings were inoculated in the basic propagation medium to propagate the initial seedlings. The basic propagation medium was: MS powder 4.43g / L+sucrose 60g / L+agar 6g / L, pH 5.6-5.7. The temperature of the culture room is 25±2°C, the light intensity is 1500 lux, and the light time is 12 hours / day.

[0039] (3) Select 0.5-0.8cm diameter sub-balls for the remaining part of the test-tube seedlings, gently cut off the roots, stems, and leaves with a scalpel, and then use the sub-balls as cue balls for subse...

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Abstract

The invention discloses a lilium oriental hybrid tissue culture seedling strengthening method which comprises the following steps: taking a squamous bulb of a lilium oriental hybrid aseptic seedling, and carrying out sprout induction culture on the squama in an induction culture medium; and after the sprouts are induced, inoculating the sprouts to a rooting and bulb-strengthening culture medium, and culturing to obtain a tissue culture seedling, wherein the rooting and bulb-strengthening culture medium comprises 4-5 g / L MS (Murashige-Skoog) powder, 60-70 g / L sucrose, 6-8 g / L agar and 0.2-20 mg / L humic acid, and the fulvic acid content in the humic acid is at least 90%. The method can promote the expansion or / and weight gain of the squamous bulb of the tissue culture seedling, thereby obtaining the strong tissue culture seedling, shortening the production cycle and saving the cost.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a method for tissue-cultivating strong seedlings of Oriental lily. Background technique [0002] Humic acid (Humic Acid, HA) is widely present in nature. It is an organic matter formed by the remains of organisms (mainly plants) through microbial decomposition and a series of geochemical processes. It is a type of natural organic matter with complex components. Due to its complex structure, HA belongs to an amorphous polymer compound. Usually, the molecular weight of HA refers to its average molecular weight. [0003] In 1919, Oden proposed to divide HA into water-soluble fulvic acid, ethanol-soluble brown humic acid and alkali-soluble black humic acid. At present, humic acid has been widely used in various fields such as agriculture, forestry, animal husbandry, petroleum, chemical industry, building materials, medicine and health, environmental protection, etc., and du...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴昀夏宜平沈柏春常乐张佳平佘琳芳王冠群
Owner HANGZHOU LANDSCAPING
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