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A method for remediation of polycyclic aromatic hydrocarbon-contaminated farmland soil by strengthening ryegrass and rhizosphere microorganisms with anionic-non-mixed surfactants

A technology of surfactants and polycyclic aromatic hydrocarbons, applied in the field of contaminated soil remediation, can solve the problems of low bioavailability and limited bioremediation efficiency, and achieve the effects of promoting the absorption and accumulation of PAHs, improving remediation efficiency, and simple technical processes

Active Publication Date: 2015-08-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of bioremediation of organically polluted soil, microorganisms and plants usually can only absorb and degrade dissolved organic pollutants in the soil solution, while hydrophobic organic pollutants have low water solubility and are usually strongly adsorbed on the soil, microorganisms and The low bioavailability of plants greatly limits the efficiency of bioremediation [Document 1: Chen B L, Johnson E J, Chefetz B, Zhu L Z, Xing B S, 2005. Sorption of polar and nonpolar aromatic organic contaminants by plant cuticular materials: role of polarity and accessibility. Environmental Science and Technology, 39(16): 6138--6146. Literature 2: Collins C, Fryer M, Grosso A, 2006. Plant uptake of nonionic organic chemicals. Environmental Science and Technology, 40(1 ): 45--52]

Method used

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  • A method for remediation of polycyclic aromatic hydrocarbon-contaminated farmland soil by strengthening ryegrass and rhizosphere microorganisms with anionic-non-mixed surfactants
  • A method for remediation of polycyclic aromatic hydrocarbon-contaminated farmland soil by strengthening ryegrass and rhizosphere microorganisms with anionic-non-mixed surfactants
  • A method for remediation of polycyclic aromatic hydrocarbon-contaminated farmland soil by strengthening ryegrass and rhizosphere microorganisms with anionic-non-mixed surfactants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Weigh about 500 g (dry weight) of contaminated soil onto a clean plastic film (40 cm in length and width). Further, 60 mL of SDBS-Tween 80 anion-nonionic mixed surfactant with a mass concentration of 10 mg / kg (concentration × volume) was added to the soil, wherein, SDBS: Tween 80 = 0:5 (mass ratio). Mix the soil and surfactant thoroughly, then put it on a clean non-woven fabric, adjust the field water holding capacity with a soil moisture content of about 60%, and finally, fully pulverize the soil and put it into a series of pots (diameter 12 cm× 10 cm high).

[0032] After the ryegrass seeds are screened and induced to become white, 8 clusters are planted in each pot, with 5 plants in each cluster. Pots are placed randomly on long shelves in the greenhouse. During the potting period, the temperature in the greenhouse is 28-32°C during the day and 20-25°C at night. Pots were watered as needed to maintain field capacity at a soil moisture content of 60%. After 40...

Embodiment 2

[0034] Weigh about 500 g (dry weight) of contaminated soil onto a clean plastic film (40 cm in length and width). Further, 60 mL of SDBS-Tween 80 anion-nonionic mixed surfactant with a mass concentration of 10 mg / kg (concentration × volume) was added to the soil, wherein, SDBS: Tween 80 = 1:4 (mass ratio). Mix the soil and surfactant thoroughly, then put it on a clean non-woven fabric, adjust the field water holding capacity with a soil moisture content of about 60%, and finally, fully pulverize the soil and put it into a series of pots (diameter 12 cm× 10 cm high).

[0035] After the ryegrass seeds are screened and induced to become white, 8 clusters are planted in each pot, with 5 plants in each cluster. Pots are placed randomly on long shelves in the greenhouse. During the potting period, the temperature in the greenhouse is 28-32°C during the day and 20-25°C at night. Pots were watered as needed to maintain field capacity at a soil moisture content of 60%. Afte...

Embodiment 3

[0037] Weigh about 500 g (dry weight) of contaminated soil onto a clean plastic film (40 cm in length and width). Further, 60 mL of SDBS-Tween 80 anion-nonionic mixed surfactant with a mass concentration of 10 mg / kg (concentration × volume) was added to the soil, wherein, SDBS: Tween 80 = 2:3 (mass ratio). Mix the soil and surfactant thoroughly, then put it on a clean non-woven fabric, adjust the field water holding capacity with a soil moisture content of about 60%, and finally, fully pulverize the soil and put it into a series of pots (diameter 12 cm× 10 cm high).

[0038] After the ryegrass seeds are screened and induced to become white, 8 clusters are planted in each pot, with 5 plants in each cluster. Pots are placed randomly on long shelves in the greenhouse. During the potting period, the temperature in the greenhouse is 28-32°C during the day and 20-25°C at night. Pots were watered as needed to maintain field capacity at a soil moisture content of 60%. Afte...

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Abstract

The invention discloses a method for restoring soil in a farmland polluted by polycylic aromatic hydrocarbons (PAHs) by anionic-nonionic mixed surface active agent enhanced ryegrass and rhizospheric microorganisms and belongs to the technical field of polluted soil restoration. The method comprises the following steps of (1) applying a low-concentration (30-150mg / kg) anionic-nonionic mixed surface active agent to the surface layer of PAHs polluted soil; (2) planting the ryegrass, harvesting plant bodies after the ryegrass grows for 40-60 days, and burning the plant bodies after the plant bodies are dried and transferred in a concentrative manner; (3) repeating the operation to clear away the PAHs in the polluted soil until the soil meets an environment safety standard. The method has the advantages that the low-dosage anionic-nonionic mixed surface active agent is adopted to elute the PAHs adsorbed on the soil in a solubilizing manner, so that the rhizospheric microorganisms can be promoted to degrade the PAHs, and the PAHs absorbed and accumulated by the plants can be improved; therefore, the efficiency for restoring the PAHs is improved. The method is simple in technology, low in cost, practical and safe.

Description

technical field [0001] The invention relates to the technical field of remediation of polluted soil, in particular to a method for remediating farmland soil polluted by polycyclic aromatic hydrocarbons by strengthening ryegrass and rhizosphere microorganisms with negative-non-mixed surfactants. Background technique [0002] PAHs (polycyclic aromatic hydrocarbons, referred to as PAHs in English, and polycyclic aromatic hydrocarbons in Chinese) are typical persistent organic pollutants commonly found in soil environments, and their concentration levels have risen from μg / kg to mg / kg. The detection rate rose from less than 20% to more than 80%, and the PAHs exceeding standard rate of major agricultural products was about 20%. Therefore, the remediation of farmland soil contaminated by persistent organic compounds such as PAHs has become one of the hot issues of common concern in soil and environmental science circles at home and abroad. However, there is still a lack of econ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/08
Inventor 朱利中倪贺伟周文军
Owner ZHEJIANG UNIV
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