Three biocontrol bacterial strains for controlling black spot disease of Xinjiang red date tree
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A technology of bacteria and jujube, applied in the field of three antagonistic bacterial strains for the prevention and control of black spot disease of jujube trees in Xinjiang, can solve problems such as lag in comprehensive management level
Inactive Publication Date: 2014-04-02
XINJIANG AGRI UNIV
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With the application of jujube direct seeding technology, the planting scale of jujube has expanded rapidly, and the dwarf and dense planting cultivation mode has been widely adopted in Xinjiang, but the comprehensive management level is relatively lagging behind, resulting in black spot, fruit shrinkage, cracking and other diseases in some areas. Severe occurrence, especially date black spot
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Embodiment 1
[0012] The soil dilution method was used to screen and isolate the bacteria, and it was identified as Microbacillus according to the morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis. The strain is derived from the soil. The shape of the 012 bacteria is rod-shaped, and the flagella characteristics are not obvious. It is a Gram-negative bacteria. The single colony is nearly round, milky white, creamy, opaque, with raised wrinkles in the center, and the edges are neat. Difficult to emulsify; the shape of 045-1 bacteria is short rod-shaped, flagellum features are not obvious, it is a Gram-positive bacteria, the single colony is nearly round, milky white, creamy, opaque, with raised wrinkles in the center and neat edges , easy to emulsify; the shape of 063-1 bacteria is short rod-shaped, and the characteristics of flagella are not obvious, which is a Gram-positive bacteria. The single colony is nearly round, milky white, creamy, opaque, with ...
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Abstract
The invention discloses three biocontrol bacterial strains for controlling a black spot disease of a Xinjiang red date tree. The bacterial strains belong to bacillus. The bacillus is screened through a plate antagonism experiment to effectively inhibit growth of black spot pathogenic bacteria of the red date tree. The bacterial strains are from soil and include 012 thallus, 045-1 thallus and 063-1 thallus, wherein the 012 thallus is rod-shaped bacterium with unconspicuous flagellum and is Gram negative bacterium; the single colony is round-like, milk white, butyrous, nontransparent, uniform in the edge, and difficult to emulsify, and has ridgy ruga in the center; the 045-1 thallus is rod-shaped bacterium with unconspicuous flagellum and is Gram positive bacterium; the single colony is round-like, milk white, butyrous, nontransparent, uniform in the edge, and easy to emulsify, and has ridgy ruga in the center; the 063-1 thallus is rod-shaped bacterium with unconspicuous flagellum and is Gram positive bacterium; the single colony is round-like, milk white, butyrous, nontransparent, uniform in the edge, and difficult to emulsify, and has ridgy wrinkles in the center. As a biological agent, the bacterial strains are free of harmful ingredients of chemical pesticides, free of toxicity and pollution, easy to produce, and wide in application prospect.
Description
technical field [0001] The invention belongs to the field of forest protection, and specifically relates to three antagonistic bacterial strains for preventing and treating the black spot disease of Xinjiang jujube trees and applications thereof. Background technique [0002] With the deepening of people's understanding of the nutritional value and medicinal value of red dates, the increase in social demand has effectively stimulated the enthusiasm for large-scale development and industrialized management of jujube trees in Xinjiang. new industries. With the adjustment of the agricultural industry structure and the implementation of the policy of returning farmland to forests, the jujube industry in Xinjiang has developed rapidly. By the end of 2009, the planting area of jujube in Xinjiang had reached 320,700 hm 2 , Accounting for about 21.38% of the country, southern Xinjiang has become the production base of my country's commodity jujube. With the application of jujube...
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