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Application of co-blocking of PD-1 and TIM-3 signal paths to anti-stomach-cancer treatment

A technology of TIM-3 and signaling pathways, applied in the field of biotechnology and medicine, can solve problems such as lack of integrity and deficiency

Inactive Publication Date: 2014-04-16
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies lacked a holistic study of PD-1 and TIM-3 in peripheral PBMCs and TILs of cancer patients CD3 + , CD4 + and CD8 + Expression levels in T cells, and the lack of expression of CD4 after co-blocking PD-1 and TIM-3 signaling pathways + and CD8 + Evaluation of effects on T cell activity

Method used

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  • Application of co-blocking of PD-1 and TIM-3 signal paths to anti-stomach-cancer treatment
  • Application of co-blocking of PD-1 and TIM-3 signal paths to anti-stomach-cancer treatment
  • Application of co-blocking of PD-1 and TIM-3 signal paths to anti-stomach-cancer treatment

Examples

Experimental program
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Effect test

Embodiment

[0054] Example: Co-blocking PD-1 and TIM-3 signaling pathways on the effect of gastric cancer cells cultured in vitro Killing effect

[0055] (1) Materials

[0056] 1. Specimens and cell lines

[0057] 1) Gastric cancer tissue, paracancerous tissue and peripheral blood of gastric cancer patients (provided by the First People's Hospital Affiliated to Soochow University);

[0058] 2) Peripheral blood of normal people (provided by the Second People's Hospital Affiliated to Soochow University);

[0059] 3) Gastric cancer cell line (AGS from Tang Zhongying Hematology Research Center, Soochow University).

[0060] 2. Main reagents and materials

[0061] 1) RPMI1640, DMEM high glucose medium (HyClone);

[0062] 2) Fetal bovine serum (FBS), 0.25% trypsin (HyClone);

[0063] 3) Penicillin-streptomycin for cell culture (HyClone);

[0064] 4) Ficoll-paque plus lymphocyte separation medium (GE healthcare company);

[0065] 5) 7-AAD dye (BD company);

[0066] 6) LIVE / DEAD fixed...

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Abstract

The invention relates to application of co-blocking of PD-1 and TIM-3 signal paths to anti-stomach-cancer treatment, and in particular relates to a blocking agent of the PD-1 signal path, a blocking agent of the TIM-3 signal path and application of the blocking agents together with the conventional anti-stomach-cancer treatment medicaments to preparation of a medicinal composition for resisting stomach cancer. The blocking agents or a combination of the blocking agents and the conventional anti-stomach-cancer medicaments treats stomach cancer by enhancing the stomach cancer cell killing effect of T cells. The composition has a remarkable treatment effect, and has the advantages of small toxic and side effects and cooperation with the conventional stomach cancer treatment medicaments.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and specifically relates to a new method for treating gastric cancer, which is to jointly block PD-1 and TIM-3 signaling pathways; meanwhile, it is proved that the combination of the method and existing anti-gastric cancer therapeutic drugs has a synergistic effect. Accordingly, the present invention provides the application of PD-1 signaling pathway blockers and TIM-3 signal pathway blockers or their combination with existing anti-gastric cancer therapeutic drugs in the treatment of gastric cancer. Background technique [0002] In a variety of mouse tumor models, about half of tumor-infiltrating lymphocytes (Tumor Infiltration Lymphocytes, TILs) co-express TIM-3 and PD-1, and show functional depletion, loss of proliferation and secretion of IL-2, TNF The ability of these cytokines -α and IFN-γ. Blocking PD-1 and TIM-3 signaling pathways together was found to be more effective in inhib...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61K45/00A61P35/00
Inventor 杨林宗云辉
Owner SUZHOU UNIV
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