Thermal cover plate used in nucleic acid amplification detector

Abstract

The invention relates to a thermal cover plate used in a nucleic acid amplification detector. The thermal cover plate comprises an upper cover plate and a lower press plate which are horizontally and oppositely arranged, wherein the upper cover plate and the lower press plate are fixedly connected through at least two springs; at least two probes are fixedly mounted on the lower press plate; displacement sensors capable of quantitatively measuring the displacement of the probes relative to the upper cover plate are fixedly mounted on the upper cover plate; the number of the displacement sensors is equal to that of the probes and the mounting positions of the displacement sensors correspond to those of the probes; at least one heating component and at least one temperature sensor which is capable of detecting the temperature of the lower press plate are fixedly mounted on the lower press plate. The problem that the nucleic acid reaction can be failed as the pressure cannot be controlled due to the fact that the conventional nucleic acid amplification detector is lack of a device which can accurately control the pressure is solved, and the pressure and the temperature can be accurately controlled.

Description

technical field [0001] The invention relates to the field of nucleic acid amplification and detection, in particular to a thermal cover plate used in an isothermal or temperature cycle nucleic acid amplification detector. Background technique [0002] In order to achieve efficient and reproducible controlled chain reaction of nucleic acid samples, a reaction condition with rapid and accurate temperature change is required, because the reproducible and precise control of the reaction temperature of nucleic acid samples in the sample pool directly determines the controlled chain reaction. The efficiency and reliability of the formula reaction are the key to improving the sensitivity and specificity of the nucleic acid amplification detector in the clinical disease diagnosis application. Existing nucleic acid amplification detectors include instruments for isothermal or temperature cycling, qualitative or real-time fluorescence quantitative detection, because the sample pool lo...

AI Technical Summary

Problems solved by technology

[0004] However, the existing nucleic acid amplification detectors lack a device that can accurately and repeatedly control pressure and temperature, so that when the mechanism, size, material and manufacturing errors of the sample pool change, it may lead to final nucleic acid reaction errors. oversized or even failed

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