A method for detecting fosfomycin sodium in pharmaceutical wastewater by ion chromatography

Abstract

The invention relates to a method for detecting fosfomycin sodium in pharmaceutical wastewater by ion chromatography, the method comprising the following steps: (1) Chromatographic conditions: the chromatographic column is a low-capacity hydroxide system cathodic protection column and a high-capacity anion exchange separation Column, suppressor adopts electrolytic membrane suppressor, conductivity detector, eluent is potassium salt solution; (2) to the preparation of standard solution: get the standard product of fosfomycin sodium and configure it into standard product solution with ultrapure water; (3) Pretreatment of the sample: the sample used for detection is filtered with a 0.45 μm filter membrane and then passed through a C18 small column to remove organic matter in the water sample; (4) Determination method: inject the standard solution into the ion chromatograph, record the spectrum, Determine the position of the standard peak, calculate the area of ​​the fosfomycin sodium peak, draw a standard curve according to the calculated peak area; take the test sample, inject it into an ion chromatograph, record the spectrum, and calculate the concentration of the sample according to the area of ​​the fosfomycin sodium peak. The invention has the advantages of good reproducibility, low detection limit, high sensitivity, simple operation, mild detection conditions, wide application of detection equipment and instruments, wide sources of eluent potassium salt solution, and good detection of fosfomycin sodium in pharmaceutical wastewater. With the advantages of simple configuration, it will be widely used in the detection of fosfomycin sodium in industrial wastewater.

Description

technical field [0001] The invention belongs to the field of compound detection, and more specifically relates to a method for detecting fosfomycin sodium in pharmaceutical wastewater. technical background [0002] Fosfomycin (FOM), chemical name (1R,2S)-1,2-epoxypropyl phosphate, is a new broad-spectrum antibiotic. Fosfomycin can effectively inhibit phosphoenolpyruvate transferase to inhibit the synthesis of mucopeptides, and has a killing effect on most Gram-positive bacteria and negative bacteria. At present, no cross-resistance has been found. It makes its clinical gradually The scale of industrial production is also gradually increasing. [0003] Fosfomycin is a highly polar small molecular compound with a molecular weight of 138 and no ultraviolet absorption at a wavelength greater than 220nm. Therefore, in the field of pharmaceutical analysis, the separation and analysis of fosfomycin has always been a major problem. In industrial production, it is mainly produced a...

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Problems solved by technology

This method is used to test the content of fosfomycin sodium in fosfomycin sodium pure product, this test method all has certain advantage in solution preparation, detection process, but detects fosfomycin sodium from waste water, still has very big room for improvement

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