A kind of preparation method of casein keratinase cross-linked heteropolymer film

A technology of keratinase and heteropolymer is applied in the field of preparation of casein keratinase cross-linked heteropolymer film, can solve problems such as insufficient modification effect, environmental problems, etc., achieves overcoming toxicity and inefficiency, and reduces production cost , The effect of improving water vapor permeability

Active Publication Date: 2016-05-18
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these modifications can improve the mechanical properties of keratin film formation to varying degrees, they also bring environmental problems, and the modification effect is not obvious enough.

Method used

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  • A kind of preparation method of casein keratinase cross-linked heteropolymer film
  • A kind of preparation method of casein keratinase cross-linked heteropolymer film
  • A kind of preparation method of casein keratinase cross-linked heteropolymer film

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A method for preparing casein keratinase cross-linked heteropolymer film includes the following steps:

[0035] 1) Preparation of casein and keratin solution

[0036] Preparation of casein solution: take 7g casein, 92g water and 1g5mol / L NaOH solution, immerse casein in part of deionized water, add NaOH solution, mix well, and soak for more than 24 hours (continuously during this process) Add part of the deionized water until the casein is completely soaked and the pH of the system has been kept below 7.0); stir the casein at this time evenly, and add the remaining deionized water. Adjust the pH value of the solution to 7.5, continue magnetic stirring until completely dissolved, and obtain a casein solution with a mass fraction of 7%;

[0037] Preparation of keratin solution: Add 7g of keratin powder into 93g of deionized water, mix well, adjust the pH of the system to 9.0 with solid NaOH, and stir at 50°C for 3 hours until the keratin is completely dissolved. The mass fracti...

Embodiment 2

[0045] A method for preparing casein keratinase cross-linked heteropolymer film includes the following steps:

[0046] 1) Preparation of casein and keratin solution

[0047] Preparation of casein solution: take 7g casein, 92g water and 1g5mol / L NaOH solution, immerse casein in part of deionized water, add NaOH solution, mix well, and soak for more than 24 hours (continuously during this process) Add part of the deionized water until the casein is completely soaked and the pH of the system has been kept below 7.0); stir the casein at this time evenly, and add the remaining deionized water. Adjust the pH value of the solution to 7.5, continue magnetic stirring until completely dissolved, and obtain a casein solution with a mass fraction of 7%;

[0048] Preparation of keratin solution: add 7g of keratin powder to 92.5g of deionized water, mix well, add 0.5g of solid NaOH to adjust the pH of the system to 9.0, magnetically stir at 45°C for 4h until the keratin is completely dissolved. ...

Embodiment 3

[0056] A method for preparing casein keratinase cross-linked heteropolymer film includes the following steps:

[0057] 1) Preparation of casein and keratin solution

[0058] Preparation of casein solution: take 7g casein, 92g water and 1g5mol / L NaOH solution, immerse casein in part of deionized water, add NaOH solution, mix well, and soak continuously for more than 12h (in this process, continue to add Partially deionized water until the casein is completely soaked and the pH of the system has been kept below 7.0); stir the casein at this time evenly, and add the remaining deionized water. Adjust the pH value of the solution to 6.5, continue magnetic stirring until it is completely dissolved, and obtain a casein solution with a mass fraction of 7%;

[0059] Preparation of keratin solution: Add 7g of keratin powder into 92.5g of deionized water. After mixing, add 0.5g of solid NaOH to adjust the pH of the system to 9.0. At 55℃, magnetically stir for 0.5h until the keratin is complete...

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Abstract

The invention discloses a preparation method of a casein keratinase crosslinked polymer membrane, and belongs to the technical field of leather working. The method comprises the following steps: 1) mixing a casein solution with a keratin solution, and then adjusting the pH value of the mixed solution to 6.5-7.5, stewing to obtain a protein mixed solution; 2) adding glutamine transaminase transferase to the mixed solution according to the adding amount of 10-30U of transferase in each gram of dry matter protein, carrying out crosslinking reaction after fully and evenly mixing, and then inactivating, so as to obtain a mixed hybrid polymer of casein and keratin; 3) adding a film-forming agent to the mixed hybrid polymer of casein and keratin, and then molding, so as to obtain the casein keratinase crosslinked polymer membrane. The casein and the keratin are crosslinked under the catalytic action of the glutamine transaminase transferase, so as to form a covalent crosslinked macromolecule polymer. The crosslinked macromolecule polymer is improved in mechanical property, strong in water-resisting property, and good in biocompatibility, can be completely biodegraded, and has a broad application prospect in the fields of a zoology coating and a biological membrane.

Description

Technical field [0001] The invention belongs to the technical field of leather processing, and specifically relates to a preparation method of a casein keratinase cross-linked heteropolymer film. Background technique [0002] Casein is one of the most widely used film-forming agents in leather finishing, and it is green and pollution-free. As a leather finishing agent, it has strong adhesion, not easy to fall off due to friction, good temperature resistance, light resistance, and good It has the advantages of air permeability and water vapor permeability, but the casein coating also has disadvantages such as being intolerant to wet rubbing, brittle and hard, easy to break when bending, poor corrosion resistance, and easy to mold. At home and abroad, caprolactam-modified casein and vinyl monomer graft copolymerization-modified casein have been industrialized. In addition, some people have studied the modification of casein with silicone, polyurethane, and melamine resin. However,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J5/18C12P21/02C08L89/00
Inventor 王鸿儒苏小舟黄勉
Owner SHAANXI UNIV OF SCI & TECH
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