43 results about "Glutamine transferase" patented technology

A preparation method used for preparing a compound protein foaming agent belongs to the food biotechnology field. The method adopts isolated soy protein, wheat gluten protein, rice protein and whey protein as materials which are respectively hydrolyzed properly by alcalase; then the hydrolyzates are mixed by a certain proportion and the mixed hydrolyzate is hyperfiltrated to obtain protein peptide provided with the molecular weight higher than 10kDa; a hydrophobic classification method is adopted to isolate the peptide to obtain the peptides of different types of hydrophobicity; then the peptides are crossly linked with TGase for recombination; the linked production is condensed and frozen for drying to obtain the compound protein foaming agent. The invention relates to the modification to structure and functional property of protein and the frothing capability and froth stability of the prepared product are both better than that of egg white protein and the frothing capability is enhanced by 10 to 30 percent compared with egg white protein. The invention strongly promotes the extensive application of the natural food foaming agent to the food production and largely enhances the availability and added value of various low-value protein resources with excellent economic benefit.

The invention discloses a preparation method of a drug-loaded sodium alginate / gelatin composite hydrogel band-aid. The method comprises the following steps: (1) mixing sodium alginate and a gelatin solution, and preparing sodium alginate / gelatin composite hydrogel by utilizing the characteristics of cross-linking between calcium ions and sodium alginate and cross-linking between glutamine transferase and gelatin; and (2) preparing the drug-loaded sodium alginate / gelatin composite hydrogel band-aid: loading a drug into the composite hydrogel, so that the finally prepared band-aid has the effects of resisting bacteria, diminishing inflammation and promoting wound healing. The drug-loaded sodium alginate / gelatin composite hydrogel band-aid is successfully prepared, the raw materials adopted in the preparation process are all non-toxic compounds, the preparation process is simple, and the cost is low. Experimental tests show that the composite hydrogel is of a porous structure and is beneficial to gas exchange; the swelling property is beneficial to absorption of exudate of the wound; and berberine serving as a drug model is successfully loaded into the composite hydrogel and can be slowly released, so that the composite hydrogel has an antibacterial effect.

A processed livestock meet food or a processed sea food having improved physical properties and eating-quality can be produced by using an enzyme having a glycosyl transfer activity for converting an a-1,4 bond to an a-1,6 bond and a transglutaminase in the process for producing the processed livestock meet food or the processed sea food.

The invention discloses a method for improving gel property of minced fish product through adding glutamine aminotransferase which comprises, adding into minced fish processed from fresh fish as raw material complex addition agent consisting soybean protein, polyphosphates and glutamine aminotransferase, adding addition agent, then kneading the minced fish 10-20 minutes, the gel property of the obtained minced fish product is increased to 400-500g from the previous 200g before addition.

The invention discloses a preparation method for an edible enzyme-modified chitosan / walnut protein composite film, and belongs to the technical field of food packaging. The method comprises the following steps: preparing a 5-7% walnut protein solution, performing full stirring, adding 3-5% of chitosan, performing uniform mixing, adding 2-3% of glycerol, performing mixing, adding 0.08-0.10% of glutaraldehyde, performing mixing, adding transglutaminase with 5 U / g protein-25 U / g protein, adjusting the pH to be 7-9, performing heating in a water bath under the temperature condition of 40-60 DEG Cfor 1-5 h, after the reaction is completed, performing enzyme deactivation under the temperature condition of 85 DEG C, performing cooling, performing ultrasonic treatment at power of 200 W for 15 min, and performing filtration to remove air bubbles; and pouring the filtered film solution into a flatly-placed container, performing air blast drying in a drying oven at the temperature of 60 DEG C for 3 h, after drying is finished, putting the dried material in an environment with relative humidity of 50% and temperature of 25 DEG C, performing equilibration for 48 h, and after equilibration is completed, taking the film. The composite film obtained by the method provided by the invention has a tensile strength of about 19 MPa, an elongation at break of 80% or more, a water vapor transmissioncoefficient of 4.245 g*mm / (m<2>*d*kPa), and a certain antibacterial effect.

The invention relates to selective diagnosis of gluten-induced autoimmune diseases by binding assays utilizing the main celiac epitope present on proteins of the transglutaminase family.

The use for a whole muscle product of a preparation containing calcium hydroxide and / or calcium oxide, and trisodium citrate and / or tripotassium citrate in a specific ratio improves the hardness significantly, as well as the water retention and the bindability, providing no troubles in appearance, even without the addition of a phosphate or with a reduced amount of the addition of a phosphate, and the properties thereof are close to those obtained by using a phosphate. <??>The properties much closer to those obtained by using a phosphate are demonstrated by means of using a transglutaminase together with the preparation, resulting from the synergistic increase in hardness of the whole muscle product with an extremely small amount of the addition of a transglutaminase. Further, the increase, with time, in the viscosity of a pickle due to the transglutaminase is significantly prevented by controlling the pH of the pickle within a specific range.

The invention discloses a preparation method of a casein keratinase crosslinked polymer membrane, and belongs to the technical field of leather working. The method comprises the following steps: 1) mixing a casein solution with a keratin solution, and then adjusting the pH value of the mixed solution to 6.5-7.5, stewing to obtain a protein mixed solution; 2) adding glutamine transaminase transferase to the mixed solution according to the adding amount of 10-30U of transferase in each gram of dry matter protein, carrying out crosslinking reaction after fully and evenly mixing, and then inactivating, so as to obtain a mixed hybrid polymer of casein and keratin; 3) adding a film-forming agent to the mixed hybrid polymer of casein and keratin, and then molding, so as to obtain the casein keratinase crosslinked polymer membrane. The casein and the keratin are crosslinked under the catalytic action of the glutamine transaminase transferase, so as to form a covalent crosslinked macromolecule polymer. The crosslinked macromolecule polymer is improved in mechanical property, strong in water-resisting property, and good in biocompatibility, can be completely biodegraded, and has a broad application prospect in the fields of a zoology coating and a biological membrane.

A process for producing a non-dairy food product of enhanced viscosity comprises providing a mixture of deamidated oat base and vegetable protein isolate, crosslinking of glutamine and lysine units ofthe protein isolate and oat protein comprised by the oat base by means of transglutaminase, and fermenting the mixture by means of a yogurt, sour cream or cheese starter culture. Also disclosed are products obtainable by the process and their use.

Fluorescent probe and its preparation method and use. The fluorescent probe has the structure of formula I. The fluorescent probe molecule of the above formula I structure of the present invention is a long-wavelength fluorescent probe with excellent two-photon performance designed based on the intramolecular charge transfer (Intramolecular Charge Transfer, ICT for short) mechanism. By targeting the overexpressed γ-glutamine transferase on the surface of cancer cells, the recognition site is selectively cleaved by the enzyme, thereby releasing a strong electron-donating group and enhancing the fluorescence at long wavelengths. This fluorescent probe has the advantages of good solubility, strong photostability, not affected by the acidity and alkalinity of the system, and two-photon excitation (excitation wavelength 800nm), and can realize selective confocal fluorescence imaging of cancer cells .