Fluorescence probe and preparation method and application thereof

A technology of fluorescent probes and detection reagents, which is applied in the field of preparation of fluorescent probes for distinguishing normal cells and cancer cells, can solve the problems of expensive instruments, short excitation wavelengths, complicated pretreatment processes, etc., and achieves strong light stability, good The effect of solubility
CN107793386AActive Publication Date: 2018-03-13DALIAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
DALIAN UNIV OF TECH
Publication Date
2018-03-13

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Abstract

Disclosed are a fluorescence probe and a preparation method an application thereof. The fluorescence probe is of a structure as shown in the formula I, which is a long-wavelength fluorescence probe with excellent two-photon performance and designed on the basis of intramolecular Charge Transfer (TCT) mechanism. By means of gamma-transglutaminase which is in over-expression on the surface of targeted cancel cells, recognition sites are cut off by enzyme selectively, strong electron-donating groups are then released, and thus long-wavelength fluorescent light is strengthened. The fluorescence probe has excellent solubility and high light stability, is free of influence from acidity and basicity, is featured by double-photon laser, and the like. Besides, the fluorescence probe realizes confocal fluorescence imaging selectively to cancel cells.
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Description

Technical field

[0001] The invention belongs to the field of fine chemicals, and relates to a preparation method and application of a fluorescent probe for distinguishing normal cells from cancer cells. Background technique

[0002] With the deterioration of the living environment and the increase of life pressure, the number of humans suffering from cancer is showing a trend of rapid increase. According to statistics, there were 14.1 million new cancer patients worldwide in 2015, and the death toll was as high as 8.2 million. It is estimated that new cases will increase by 70% in the next 20 years. Due to the delay of diagnosis and the lack of effective personalized treatment plans, the inefficiency of treatment for cancer patients is as high as 75%. For an ordinary family, it often brings a catastrophic blow. Although the government invests a large amount of research funding every year in the hope of conquering the treatment of cancer. But for now, through the "early detecti...

Claims

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