Artificial bone scaffold material for enhancing biocompatibility and preparation method thereof

Abstract

The invention relates to a preparation method of an artificial bone scaffold material. In application of bone tissue engineering, a cross-linking agent is usually used to improve anti-pressure ability, degradation property and the like, of an artificial bone, but chemical cross-linking agents such as glutaraldehyde and carbodiimide are usually used at present, the chemical cross-linking agents usually have a toxicity problem and have potential safety hazard for hosts. According to the preparation method, enzyme- transglutaminase is used as a cross-linking agent to improve water solubility and mechanical strength of the prepared collagen / nano hydroxyapatite artificial bone, and inactivation treatment is carried out onto the enzyme after the reaction. In the whole preparation process, no substances or reagents which are toxic and harmful or have potential hazard are introduced, so that biocompatibility of the artificial bone material is greatly improved, the related reactants are free of biological toxicity, and possible potential safety hazards of products on body are reduced; meanwhile, osteoconduction of the hydroxyapatite is also kept, and cell production promotion performance of the collagen is not affected.

Description

technical field [0001] The invention relates to a biomedical material, in particular to an artificial bone scaffold material with enhanced biocompatibility and a preparation method thereof. Background technique [0002] In orthopedic surgery and biomedical engineering, the preparation of bone scaffold materials with excellent performance is still one of the key issues. As a human bone implant material, the scaffold needs to provide a microenvironment similar to that of natural bone, which is conducive to cell adhesion, proliferation and differentiation, and can guide the growth of blood vessels. It has high biological activity and cannot cause immune rejection and inflammation of the body. reaction. [0003] Biocompatibility is the core factor to be considered in the preparation of scaffold materials. The factors affecting the biocompatibility of scaffold materials include not only the structure of the scaffold material itself, but also the physical and chemical propertie...

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Problems solved by technology

[0005] There have been related studies on the construction of collagen-hydroxyapatite scaffold materials, but the preparation process is cumbersome and complicated, which is not conducive to scale-up production, or the micropore diameter does not meet the corresponding technical requirements (50-150??m), and the pore diameter Relatively small, not conducive to inward climbing, growth of cells and ingrowth of vascular tissue

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