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Preparation method of in-vivo hemostatic dressing which takes transglutaminase as cross-linking agent

The technology of hemostatic dressing and glutamine, which is applied in the directions of pharmaceutical formulations, surgical adhesives, bandages, etc., can solve the problems of loss of protein biological activity, easy generation of rejection reaction, poor processability, etc. The effect of solving the hidden danger of water insolubility and virus, and high safety

Active Publication Date: 2017-05-31
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, collagen is mostly derived from animal skin, tendons, etc., and its processability is poor. During the extraction process, part of the biological activity of the protein will be lost, and it is a heterologous substance to the human body, which can cause adverse reactions such as allergies. Very prone to rejection in the human body

Method used

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  • Preparation method of in-vivo hemostatic dressing which takes transglutaminase as cross-linking agent
  • Preparation method of in-vivo hemostatic dressing which takes transglutaminase as cross-linking agent
  • Preparation method of in-vivo hemostatic dressing which takes transglutaminase as cross-linking agent

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preparation example Construction

[0026] A preparation method of an internal hemostatic dressing, comprising the steps of:

[0027] (1) Dissolve human-like collagen in water for injection, stir to dissolve it completely, so that the mass concentration of human-like collagen in the solution is 1%-5%. A human-derived collagen produced by high-density fermentation;

[0028] (2) Transfer the solution into disposable sterile Petri dishes or molds, 7-15 mL per dish or mold;

[0029] (3) Add transglutaminase, the ratio of transglutaminase to human-like collagen is 4-8U / g, the reaction temperature is -4-0°C, and the cross-linking time is 24-48 hours;

[0030] (4) During the pre-freezing process, use the program freezer to control the cooling rate at 4-8°C / min for precise cooling down to -40--80°C;

[0031] (5) Freeze-dry the sample in a vacuum freeze dryer for 24-72 h;

[0032] (6) Freeze-dried samples are sterilized by irradiation for 10-30 h.

[0033] The present invention uses human-like collagen as the main ra...

Embodiment 1

[0036] Dissolve human-like collagen in water for injection, after dissolving, add transglutaminase, stir to make it dissolve completely, make the mass concentration of human-like collagen in the mixed solution be 2.0%, the mass concentration of glutaminase 4U per gram of protein, placed in a -4°C refrigerator for cross-linking reaction for 24 hours, frozen in a programmed freezer at a rate of -4°C / min to -80°C, and kept for 2 hours, then quickly transferred to a vacuum freeze dryer for freezing After drying for 24 hours, take out the package and sterilize it with Co60 radiation for 24 hours to obtain a hemostatic dressing in the body. The prepared sponge has a porosity of 92% and a water absorption rate of 1500%.

Embodiment 2

[0038] Dissolve human-like collagen in water for injection, after dissolving, add transglutaminase, stir to make it dissolve completely, make the mass concentration of human-like collagen in the mixed solution be 2.5%, the mass concentration of glutaminase 4U per gram of protein, placed in a -4°C refrigerator for cross-linking reaction for 30 hours, frozen in a programmed freezer at a rate of -4°C / min to -80°C, and kept for 2 hours, then quickly transferred to a vacuum freeze dryer for freezing After drying for 30 h, the packaging was taken out and sterilized by Co60 irradiation for 24 h to obtain a hemostatic dressing in the body.

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Abstract

The invention discloses a preparation method of an in-vivo hemostatic dressing. The preparation method comprises the following steps: after adding the transglutaminase in a human-like collagen solution for cross linking, accurately cooling and pre-freezing through a set cooling speed, carrying out vacuum freeze drying, then carrying out Co60 irradiation sterilizing, and finally obtaining the in-vivo hemostatic dressing. The in-vivo hemostatic dressing prepared by the preparation method disclosed by the invention is high in safety, good in biocompatibility and remarkable in hemostatic effect, can be used for all kinds of wound hemostasis and postoperation hemostasis and has a good application prospect in related fields of medical biomaterials, tissue engineering and the like.

Description

technical field [0001] The invention relates to a preparation method of an in vivo hemostatic dressing, in particular to a preparation method of an in vivo hemostatic dressing using transglutaminase as a crosslinking agent, and belongs to the field of biomedical materials. Background technique [0002] Collagen is a high-molecular protein that is generally white, opaque, and fibrous. It is one of the main components of the structure of organisms and is widely distributed in the skin, bones and muscle tissues of animals. Collagen has hemostatic properties. On the one hand, it can strengthen the adhesion and aggregation of platelets to form thrombus and prevent blood from flowing out. On the other hand, the intrinsic coagulation pathway can also be initiated by activating and inducing various coagulation factors. However, collagen is mostly derived from animal skin, tendons, etc., and its processability is poor. During the extraction process, part of the biological activity ...

Claims

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Application Information

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IPC IPC(8): A61L26/00A61L24/10A61L24/00
CPCA61L24/0036A61L24/102A61L26/0033A61L26/0085A61L2400/04C08L89/00
Inventor 范代娣惠俊峰米钰马晓轩马沛刘琳
Owner NORTHWEST UNIV
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