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Method for identifying malignant pleural effusion

A malignant pleural effusion and pleural effusion technology, applied in the field of malignant pleural effusion detection, can solve problems such as correlations that have not yet been seen, and achieve the effect of reducing the interference of human subjective factors, high sensitivity, and good specificity

Active Publication Date: 2014-04-30
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there is no research report on the correlation between CD163 molecule and malignant pleural effusion and its application in identifying malignant pleural effusion

Method used

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  • Method for identifying malignant pleural effusion
  • Method for identifying malignant pleural effusion
  • Method for identifying malignant pleural effusion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] In order to test the accuracy of the detection of malignant pleural effusion provided by the present invention, the inventors conducted the following comparative experiments.

[0026] Source of experimental specimens:

[0027] The specimens came from patients in the Department of Oncology and Respiratory Department of the First Affiliated Hospital of Zhengzhou University, a total of 100 cases. Among them, 50 cases of malignant pleural effusion were collected from patients with lung cancer, and 50 cases of inflammatory pleural effusion were collected from patients with inflammation. The pleural effusions of all patients were confirmed by cytological examination of the pathology department. The clinical data and analysis statistics of the patients are shown in the table below. The samples were collected after obtaining the consent of the patients, and an informed consent was signed.

[0028] Clinical data of patients with inflammatory pleural effusion and malignant ...

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Abstract

The invention belongs to the technical field of malignant pleural effusion detection methods, and specifically relates to a method for identifying the malignant pleural effusion by using CD14+CD163+cells. The method comprises the following steps of extracting mononuclear cells, labeling antibodies, and performing immunophenotyping analysis by using a flow cytometry. Compared with the prior art, the method provided by the invention has the advantages that the method is simple and convenient and rapid, the sensitivity is high, the specificity is high, meanwhile, the interference of man-made subjective factors is reduced by using the method, the method is objective, judgment and identification can be made for the potential malignant pleural effusion patient as soon as possible, and convenience is provided for the subsequent in-time intervention treatment.

Description

technical field [0001] The invention belongs to the technical field of detection methods for malignant pleural effusion, and in particular relates to a method using CD14 + CD163 + A method for cellular identification of malignant pleural effusions. Background technique [0002] Pleural effusion is a common clinical presentation. There are many reasons for pleural effusion, including malignant pleural effusion caused by direct invasion of tumor cells or pleural metastasis caused by bloody diffusion, and other benign causes, such as inflammatory pleural effusion caused by tumor complicated with heart failure, tuberculosis, etc. Effusion. Malignant pleural effusion is a common clinical manifestation of tumor patients, so the judgment of benign and malignant pleural effusion is closely related to treatment and prognosis. [0003] Currently, clinical methods for diagnosing and identifying pleural effusions include clinical manifestations, thoracentesis test results, pleural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/57488G01N33/6893G01N2333/535G01N2800/7095
Inventor 张毅王菲
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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